Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 294529..295172 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP081768 | ||
| Organism | Escherichia coli strain 0H18 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | - |
| Locus tag | J6O59_RS26095 | Protein ID | WP_088765932.1 |
| Coordinates | 294529..294945 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | J6O59_RS26100 | Protein ID | WP_001261282.1 |
| Coordinates | 294942..295172 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J6O59_RS26050 (J6O59_25900) | 289980..290744 | + | 765 | WP_000076190.1 | plasmid replication initiator RepA | - |
| J6O59_RS26055 (J6O59_25905) | 291317..291559 | + | 243 | WP_000124732.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| J6O59_RS26060 (J6O59_25910) | 291552..291836 | + | 285 | WP_000277490.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| J6O59_RS26065 (J6O59_25915) | 291974..292066 | - | 93 | Protein_324 | plasmid partition protein ParG | - |
| J6O59_RS26070 | 292386..292604 | - | 219 | Protein_325 | IS110 family transposase | - |
| J6O59_RS26075 (J6O59_25920) | 292605..292904 | - | 300 | Protein_326 | glycogen/starch synthase | - |
| J6O59_RS26080 (J6O59_25925) | 292904..293425 | - | 522 | Protein_327 | glucose-1-phosphate adenylyltransferase | - |
| J6O59_RS26085 (J6O59_25930) | 293488..294192 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| J6O59_RS26090 (J6O59_25935) | 294291..294431 | + | 141 | WP_045286941.1 | helix-turn-helix domain-containing protein | - |
| J6O59_RS26095 (J6O59_25940) | 294529..294945 | - | 417 | WP_088765932.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| J6O59_RS26100 (J6O59_25945) | 294942..295172 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| J6O59_RS26105 (J6O59_25950) | 295129..295590 | + | 462 | WP_223584524.1 | hypothetical protein | - |
| J6O59_RS26110 (J6O59_25955) | 295734..296084 | + | 351 | WP_000493399.1 | hypothetical protein | - |
| J6O59_RS27200 | 296128..296250 | + | 123 | WP_257606738.1 | hypothetical protein | - |
| J6O59_RS26115 (J6O59_25960) | 296244..297041 | - | 798 | WP_000544830.1 | IS21-like element IS21 family helper ATPase IstB | - |
| J6O59_RS26120 (J6O59_25965) | 297041..298213 | - | 1173 | WP_042111630.1 | IS21-like element IS21 family transposase | - |
| J6O59_RS26125 (J6O59_25970) | 298299..298478 | + | 180 | Protein_337 | hypothetical protein | - |
| J6O59_RS26130 (J6O59_25975) | 298530..299241 | - | 712 | Protein_338 | IS6 family transposase | - |
| J6O59_RS26135 (J6O59_25980) | 299310..299750 | + | 441 | Protein_339 | ISNCY family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..303843 | 303843 | |
| - | inside | IScluster/Tn | - | - | 287214..302500 | 15286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15164.56 Da Isoelectric Point: 6.4581
>T213358 WP_088765932.1 NZ_CP081768:c294945-294529 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR
Download Length: 417 bp
>T213358 NZ_CP107574:573448-573819 [Pantoea dispersa]
GTGTGGCAGGTTGAAGCAACAGATCGGTTTTGGAAATGGTTGCAGGCTCAGGATGAAGCGTTGCGTCTCGACGTGCTTGC
CGCCCTGAAGCTTCTGGCGCAGGACGGTCCGCATCTTGGCCGCCCTTTCGTTGATACCCTGATGTTATCGCGGGTGCCCA
ATATGAAAGAGCTTCGCGTGCAAAGTAAAGGTCGTCCCATCCGCAGCTTTTTTGTTTTCGATCCGCGCCGCCATGCGATT
GTGCTGTGTGCCGGCAACAAACAGGGTAAAAATCAAAAGCGTTTCTATCAGCAGATGCTTCGCATTGCCGAAACCGAATA
CCATCATCATCTGAACGCAATCGGGGAAACACATAATGAAAACCTTGGATGA
GTGTGGCAGGTTGAAGCAACAGATCGGTTTTGGAAATGGTTGCAGGCTCAGGATGAAGCGTTGCGTCTCGACGTGCTTGC
CGCCCTGAAGCTTCTGGCGCAGGACGGTCCGCATCTTGGCCGCCCTTTCGTTGATACCCTGATGTTATCGCGGGTGCCCA
ATATGAAAGAGCTTCGCGTGCAAAGTAAAGGTCGTCCCATCCGCAGCTTTTTTGTTTTCGATCCGCGCCGCCATGCGATT
GTGCTGTGTGCCGGCAACAAACAGGGTAAAAATCAAAAGCGTTTCTATCAGCAGATGCTTCGCATTGCCGAAACCGAATA
CCATCATCATCTGAACGCAATCGGGGAAACACATAATGAAAACCTTGGATGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT213358 WP_001261282.1 NZ_CP081768:c295172-294942 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT213358 NZ_CP107574:573803-574102 [Pantoea dispersa]
ATGAAAACCTTGGATGAGATGCTGGCGGCGCTGACGCCCGAACAGCAACATCAGGTCGATGAACGCGTCAGCCAGATGCG
TCTTGAATACCAGCTTTACCAGTTACGTGAGCAGATGCAGCGGACTCAGCGTCAGCAAGCGCAGGCCATGCACATTGCCC
AGTCATCTGTGGCGGCAATGGAAGCGCGTGGCGCAGAGTTAAAGATTTCCACGCTGAAGCGCTACATTGAGGCGCTGGGT
GGAGAGCTGACGCTGGGTGTAAAGATGCCGGGTGGCCAGCATGTCGACTTAATTATCTGA
ATGAAAACCTTGGATGAGATGCTGGCGGCGCTGACGCCCGAACAGCAACATCAGGTCGATGAACGCGTCAGCCAGATGCG
TCTTGAATACCAGCTTTACCAGTTACGTGAGCAGATGCAGCGGACTCAGCGTCAGCAAGCGCAGGCCATGCACATTGCCC
AGTCATCTGTGGCGGCAATGGAAGCGCGTGGCGCAGAGTTAAAGATTTCCACGCTGAAGCGCTACATTGAGGCGCTGGGT
GGAGAGCTGACGCTGGGTGTAAAGATGCCGGGTGGCCAGCATGTCGACTTAATTATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|