Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4488025..4488522 Replicon chromosome
Accession NZ_CP081767
Organism Escherichia coli strain 0H18

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J6O59_RS22065 Protein ID WP_001173343.1
Coordinates 4488025..4488198 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag J6O59_RS22070 Protein ID WP_001425728.1
Coordinates 4488226..4488522 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O59_RS22055 (4484695) 4484695..4486152 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
J6O59_RS22060 (4486389) 4486389..4487906 + 1518 WP_001295074.1 lysine--tRNA ligase -
J6O59_RS22065 (4488025) 4488025..4488198 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J6O59_RS22070 (4488226) 4488226..4488522 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J6O59_RS22075 (4488749) 4488749..4489021 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J6O59_RS22080 (4489033) 4489033..4489263 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
J6O59_RS22085 (4489444) 4489444..4491075 + 1632 WP_001216486.1 sensor histidine kinase -
J6O59_RS22090 (4491072) 4491072..4491791 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4440872..4488522 47650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T213355 WP_001173343.1 NZ_CP081767:c4488198-4488025 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T213355 NZ_CP107573:c4169099-4167789 [Pantoea dispersa]
ATGACAGAACTGGATGTCTACATGAACGGCTATCGGACAGGCGTTTTCAGCAGGGCAGCAACCGGTGCACACACCTTCAG
ATATTATGAGAGCTGGCTGAATCAGCCGGGTAGCCGACCGATATCCCTCTCCATGCCTTTGCGCCACCAGCCTTACAAGG
GCGCGGAGGTCTATAACTTTTTTGATAACCTGCTGCCTGATAACCCTGAAATTCGCGCACGTATTCTGTCTCGCTATCAG
GCCAGCTCCACCCAGCCCTTTGACTTACTGGCAAAAGTCGGCAGCGACACCATTGGTGCGCTGCAATTGTTACCGCCTGA
CAGTAAACCGCACACTGTCCGAACGATCGATTATCGCCGGTTAAGCGATAGCGAACTGGAAAAAATCCTTTCAGGCTATC
AGTCAGGCGCACCGCTTGGCATGCTGGAAGAGCTGACTGATTTTCGTATTTCACTGGCTGGCGCGCAGGAGAAAACGGCG
CTGTTACGCATTAATCAACAATGGTGCCTGCCGCAGGGTAATACGCCCACCACCCATATTTTAAAATTGCCGATTGGCAA
TATTCAGAGCCATTCTCATCGCATCGATCTTTCAGATAGCGTGGAGAATGAGTGGCTTTGTACGCTGATTGCCAGAGAAT
TTGGCCTGGCAGTTCCCCCTTGCTTTATCGTAAACACCGGGAAAATTAAAGCACTGGCCGTGGAGCGGTTTGATCGGCGT
TATGCATCGGATGGACAGTGGATCATGCGGTTACCCCAGGAAGACTTTTGTCAGGTGCTGAATGTACCCGCGGCAGGAAA
ATATGAAAGCCACGGCGGCCCGGGCATCTCTGCGATTATGGATTATCTCCTGAACTCAGTTAATGCAGAACAGGATCGAT
ATGATTTCATGAAAGCTCAGGTCCTGTTTTGGCTGCTGGCGGCCACCGATGGCCATGCCAAAAACTTTTCCATTTTTATC
GAAGCGGAAGGACGTTTTCGCCTGACGCCGTTTTACGACATTCTTTCCGTTTATCCCGTGATTGGCGGTGCGGGATTACA
CATTCGTGATGCAAAGCTGGCAATGGGACTGAACGGGAGCAAAGGCCGGAAAGCCGCCATCGAACACATTTTTCCCCGCC
ATTTTATAGAAACCGCAAAAACGACAAAATTTGCCCTGACGGCGATGGCACACATCCTCGATGAGTTCAGAGCCAGAACG
CCAGAAGTGATCCAACGCGTGCAGGAGCAAATCCCTGCAGCCTTCCCGTTACGCGTGAGCCAGACCATCCTCAATGGCTT
TAAGGCGCGGGCCGCGCGTCTGCAGCAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT213355 WP_001425728.1 NZ_CP081767:c4488522-4488226 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT213355 NZ_CP107573:c4169356-4169099 [Pantoea dispersa]
ATGAAAATCACCAGCGCCAAACAATTAAGTTCTTATCTCAAAGATAGAAGACTCACAGAGGCACTCTCGCAAGCGAAAGT
CGCCAGCAAAATTGGTATACGCCAGGATACCGTCTCGAGCTTTGAGCTACAGCCAGAAACCACCCGACTGGACACGCTGT
TTCGTATTCTCGCTGCTCTGGATCTGGAAATGGAGATAAAACCCCGTGGTTTGCCATCGGCCAAACAGGACCCGGACGGC
TGGAAGGAGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References