Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4149800..4150350 Replicon chromosome
Accession NZ_CP081767
Organism Escherichia coli strain 0H18

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag J6O59_RS20380 Protein ID WP_001160971.1
Coordinates 4149800..4150114 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J6O59_RS20385 Protein ID WP_000125566.1
Coordinates 4150117..4150350 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O59_RS20355 (4145475) 4145475..4146761 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J6O59_RS20360 (4146761) 4146761..4147750 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J6O59_RS20365 (4147747) 4147747..4148568 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J6O59_RS20370 (4148571) 4148571..4148948 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J6O59_RS20375 (4148955) 4148955..4149803 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J6O59_RS20380 (4149800) 4149800..4150114 - 315 WP_001160971.1 CcdB family protein Toxin
J6O59_RS20385 (4150117) 4150117..4150350 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6O59_RS20390 (4150436) 4150436..4150915 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
J6O59_RS20395 (4151107) 4151107..4152969 - 1863 WP_000377188.1 glutathione-regulated potassium-efflux system protein KefC -
J6O59_RS20400 (4152962) 4152962..4153492 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J6O59_RS20405 (4153603) 4153603..4154934 - 1332 WP_001183198.1 MFS transporter -
J6O59_RS20410 (4154992) 4154992..4155279 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T213351 WP_001160971.1 NZ_CP081767:c4150114-4149800 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T213351 NZ_CP107573:3064902-3065120 [Pantoea dispersa]
ATGAGCAACCCAGCCTTAACAAAAACAGACTATTTGATGCGACTGCGTCGTTGTCGCTCAATTGACACCCTTGAACGGGT
GATTGAAAAGAACAAGTATGAATTACCCGATGATGAGTTAGCGGTATTCTACTCCGCAGCCGATCACCGTCTGGCCGAGC
TGACCATGAACAAGCTCTATGACAAAGTGCCCGGATCTGTCTGGAAGTACGTCCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT213351 WP_000125566.1 NZ_CP081767:c4150350-4150117 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT213351 NZ_CP107573:3064500-3064877 [Pantoea dispersa]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATTTCTGTGTGAGAACCTGTTTGACGAAAGTATGGC
GACGCTAACCGACAGCCATCACGGCTGGGTTAATGATCCCACTTCGCCAGGCAATTTACAGCTCAACGATTTGATTGAGC
ATATCGCCTCTTTCACGATGAATTACAAAATTAAGCATGTTGAAGATGAAGCGCTGATTACGCAAATCGATGAATATCTT
GATGATACCTTTATGCTGTTTAGCAGCTATGGAATCAATACCCAGGATCTTCAACGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTTGCCGAAGAGTGCGCTTATCTCCAGCAACCCAGCCATTCCTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References