Detailed information of TA system TA213350

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3901117..3901796
• Replicon: chromosome
• Accession: NZ_CP081767
• Organism: Escherichia coli strain 0H18

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O59_RS19120
• Protein ID: WP_000854680.1
• Coordinates: 3901455..3901796 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O59_RS19115
• Protein ID: WP_000070396.1
• Coordinates: 3901117..3901434 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O59_RS19070 (3896555)	3896555..3897376	+	822	WP_000197388.1	DUF932 domain-containing protein	-
J6O59_RS19075 (3897593)	3897593..3898294	+	702	WP_000189411.1	WYL domain-containing protein	-
J6O59_RS19080 (3898335)	3898335..3898571	+	237	WP_001144031.1	protein YpjK	-
J6O59_RS19085 (3898571)	3898571..3899014	+	444	WP_001547764.1	lipoprotein YafY	-
J6O59_RS19090 (3899037)	3899037..3899504	+	468	WP_001547765.1	protein YkfB	-
J6O59_RS19095 (3899581)	3899581..3899820	+	240	WP_000194654.1	DUF905 family protein	-
J6O59_RS19100 (3899918)	3899918..3900376	+	459	WP_001548150.1	antirestriction protein	-
J6O59_RS19105 (3900392)	3900392..3900868	+	477	WP_000811693.1	RadC family protein	-
J6O59_RS19110 (3900877)	3900877..3901098	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O59_RS19115 (3901117)	3901117..3901434	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O59_RS19120 (3901455)	3901455..3901796	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O59_RS19125 (3902617)	3902617..3904083	-	1467	WP_000723924.1	hypothetical protein	-
J6O59_RS19130 (3904399)	3904399..3904788	-	390	WP_223596848.1	hypothetical protein	-
J6O59_RS19135 (3905032)	3905032..3905118	-	87	Protein_3754	capsid protein	-
J6O59_RS19140 (3905323)	3905323..3906096	+	774	WP_000355484.1	hypothetical protein	-
J6O59_RS19145 (3906157)	3906157..3906711	-	555	WP_000905043.1	site-specific DNA recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3876172..3914534	38362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T213350 WP_000854680.1 NZ_CP081767:3901455-3901796 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T213350 NZ_CP107573:2473462-2473644 [Pantoea dispersa]
ATGAAGAGCGCGACGCTGATTAAAGTGCTGCAAAAGAATGGCTGGAAGCTGGAGCGCATACGCGGCAGTCATCATCAGTT
CTCGCACCCGGATTTTGCTCATCTGATCACCGTGCCGCATCCGCAGAAAGATATGAAGACAGGCACGCTGATGCAGATTC
TGAAAGACGCCAGGCTGGATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT213350 WP_000070396.1 NZ_CP081767:3901117-3901434 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT213350 NZ_CP107573:2473684-2474076 [Pantoea dispersa]
ATGCTGTTTCCCGCTTTGGTAGAGATTGACGCTGACGGCTCGGCCAGTGGTTATTTTCCGGATGTCACCGGCTGTTATTT
TGCCGGTGATACGCCCGAGCAAACGCTACAGGATGCGCAATCTGCGCTGCATGCCCACTTTGAATTGATGGCGGAGAAAG
GCTTATTGATTCCGGAGCCGGCGCAGCACTGGCAGCAGGAATTTAGCCAGCCCGGCGTGTGGATCTACGTTGATATTGAT
GTCACCCGCTATCTGGGTAAATCCGAGCGCATCAATATCACTATGCCGCATCTGCTGATTGAGAAGATCGATAAGATGGT
CAGTAATAACGCGCGCTACAGCAGCCGCAGCCATTTTCTTGCCGAGGCGGCGCGCAAAGCGCTGCTCTCATAG

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7