Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 322300..322931 Replicon chromosome
Accession NZ_CP081767
Organism Escherichia coli strain 0H18

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag J6O59_RS01500 Protein ID WP_001259385.1
Coordinates 322300..322575 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J6O59_RS01505 Protein ID WP_000593555.1
Coordinates 322572..322931 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O59_RS01485 (317304) 317304..318371 + 1068 WP_000361470.1 HlyD family secretion protein -
J6O59_RS01490 (318368) 318368..321103 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
J6O59_RS01495 (321103) 321103..322227 + 1125 WP_001488398.1 ABC transporter permease -
J6O59_RS01500 (322300) 322300..322575 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
J6O59_RS01505 (322572) 322572..322931 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J6O59_RS01510 (323051) 323051..323452 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J6O59_RS01515 (323458) 323458..324264 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J6O59_RS01520 (324261) 324261..325025 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J6O59_RS01525 (325025) 325025..325858 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J6O59_RS01530 (325855) 325855..326799 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 299562..322931 23369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T213331 WP_001259385.1 NZ_CP081767:322300-322575 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T213331 NZ_CP107548:c1508370-1508092 [Pusillimonas sp. M17]
ATGATCAAAAGTTTTCGATGCGCTGACACTCAAGCCCTTTTTGAAGGCCGGCATGTGGCCCGGTTAGCAAATATCAAAAG
TATCGCGGAACGAAAGCTGCAAATGCTCGATAGCGCAAACACGCTTATTTTTCTGCGTTCGCCGCCAGGCAATCGACTGG
AAGCGCTCAAAGGCAATCGGATCGGACAACACAGCATCCGAATCAACGACCAATGGCGCATTTGCTTCGTTTGGACCATG
GAAGGACCTGATGGCGTCGAGATCGTCGATTACCACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT213331 WP_000593555.1 NZ_CP081767:322572-322931 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT213331 NZ_CP107548:c1508078-1507776 [Pusillimonas sp. M17]
ATGTCCAAGAATGGCATGCGTCCGGTTCACCCGGGCGAAATTCTACGAGAGGAATATCTAGCGCCGCTCGGCATGAGCGT
CAACGCCTTGTCCGCCATGCTGCACGTCCCCGCTACTCGCATGAATGAAATAGTTCGGGAGCGCCGAGGCATCACGCCCG
ATACCGCGCTGCGGCTAGCCGCACTTTTCGGCGGAGATGCAAGTTCGTGGCTGAACCTTCAGCAAACCTACGACCTGAAG
AACACGGAAGCGAAAGTCGCCAAGCTGATCGCAAAACAGATCCAGCCGCTGGAACATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References