Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 45631..46274 Replicon plasmid unnamed2
Accession NZ_CP081764
Organism Escherichia coli strain CX21

Toxin (Protein)


Gene name vagD Uniprot ID A0A6N9SI53
Locus tag J6O46_RS25455 Protein ID WP_032192044.1
Coordinates 45858..46274 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag J6O46_RS25450 Protein ID WP_001261283.1
Coordinates 45631..45861 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O46_RS25420 (J6O46_25315) 40901..41452 + 552 WP_223610888.1 hypothetical protein -
J6O46_RS25425 (J6O46_25320) 41486..42183 + 698 WP_223610889.1 IS1 family transposase -
J6O46_RS25430 (J6O46_25325) 42329..43123 - 795 WP_016240356.1 site-specific integrase -
J6O46_RS25435 (J6O46_25330) 43321..44336 - 1016 Protein_55 hypothetical protein -
J6O46_RS25440 (J6O46_25335) 44506..44811 - 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB -
J6O46_RS25445 (J6O46_25340) 44813..45031 - 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA -
J6O46_RS25450 (J6O46_25345) 45631..45861 + 231 WP_001261283.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O46_RS25455 (J6O46_25350) 45858..46274 + 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O46_RS25460 (J6O46_25355) 46310..46561 + 252 WP_105966327.1 hypothetical protein -
J6O46_RS25465 (J6O46_25360) 46642..47742 - 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
J6O46_RS25470 (J6O46_25365) 49064..50407 - 1344 WP_032192047.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..94675 94675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15113.64 Da        Isoelectric Point: 7.7809

>T213329 WP_032192044.1 NZ_CP081764:45858-46274 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLTRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATIEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T213329 NZ_CP107548:c1206359-1206069 [Pusillimonas sp. M17]
ATGAAAACGATCCACACCACCGATACGTTCGATGATTGGTTCGTCGGGTTACGCGATAGGATGGCTCAGAAGCGAATCCA
AGCAAGGATACGCCGCGCTGAGTTTGGCAACTTCGGCGACTGTGAGCCTGTGGGCGAAGGCGTCTCGGAGATGCGAATTC
ACTACGGGCCGGGCTACCGCGTGTATTTTGTACAGCGTGGCCTGGAAATTGTGATCTTGCTCGCCGGTGGCGATAAATCC
ACGCAGAGTCAGGACATCAAGACTGCACTGAATCTGGTGCAACAACTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT213329 WP_001261283.1 NZ_CP081764:45631-45861 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT213329 NZ_CP107548:c1206056-1205754 [Pusillimonas sp. M17]
ATGGGAACCATCAAACTACGCAAGTGGGATATCATCGAATACCTCAAGACCGAGGAAGATATGGTGATGTACCTGCAAGC
CTGCATGGATGAAGCGGGTGACGATGCCGCCTTCATCGCGGCGGCTCTCGGCGACATTGCCCGAGCCAAAGGCATGACGC
AACTTGCAAAGGATACGGGTCTGGGGCGCGAAAGCCTTTACAAGGCACTGTCCGGCGAGGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATGCACGCGCTCGGTATCAAGCTGCACCCTGAAGCCGCTACTCACGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9SI53

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References