Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 91809..92452 Replicon plasmid unnamed1
Accession NZ_CP081763
Organism Escherichia coli strain CX21

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag J6O46_RS24850 Protein ID WP_001044765.1
Coordinates 92036..92452 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O46_RS24845 Protein ID WP_001261282.1
Coordinates 91809..92039 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O46_RS24820 88522..88659 - 138 WP_223610884.1 hypothetical protein -
J6O46_RS24825 (J6O46_24725) 88773..89753 - 981 WP_016236303.1 IS5-like element ISKpn26 family transposase -
J6O46_RS24830 (J6O46_24730) 90057..90866 - 810 WP_000654269.1 hypothetical protein -
J6O46_RS24835 (J6O46_24735) 90884..91246 - 363 WP_000533745.1 hypothetical protein -
J6O46_RS24840 (J6O46_24740) 91430..91852 - 423 WP_162534719.1 hypothetical protein -
J6O46_RS24845 (J6O46_24745) 91809..92039 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O46_RS24850 (J6O46_24750) 92036..92452 + 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O46_RS24855 (J6O46_24755) 92526..94088 + 1563 WP_001128475.1 AAA family ATPase -
J6O46_RS24860 (J6O46_24760) 94073..95095 + 1023 WP_000361404.1 helicase UvrD -
J6O46_RS24870 (J6O46_24770) 96024..96245 + 222 Protein_79 IS5/IS1182 family transposase -
J6O46_RS24875 (J6O46_24775) 96346..96711 - 366 WP_001515713.1 hypothetical protein -
J6O46_RS24880 (J6O46_24780) 96708..97193 - 486 WP_000045622.1 conjugative transfer protein MobI(A/C) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146661 146661
- flank IS/Tn - - 88773..89753 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T213326 WP_001044765.1 NZ_CP081763:92036-92452 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T213326 NZ_CP107548:c981575-981282 [Pusillimonas sp. M17]
ATGACTGAAAAACTGACAACCTACGACCCTGCCACCGCCCTGGTCGATGATGAAGAAATCGCATTCTTCATGGCTGATGC
CTTTGAAACAGGCGATGCAGCCTATATTGCCAAGGCTCTTGGCGTGGTTGCTCGCGCCAAAGGGATGACAGAAATAGCAA
AAAAAACCGGACTTTCCCGTGAGCAGCTTTATCGGTCGTTCAGTGAGCGTGGCAATCCTACTTTGAAGACTACTCTAGCC
GTGATGCGAGCGCTTGGCGTCGATATGACGGCCAAGCCGCATGTCGCGAGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213326 WP_001261282.1 NZ_CP081763:91809-92039 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213326 NZ_CP107548:c981873-981577 [Pusillimonas sp. M17]
ATGATCGAGCTAAAGCAGACAAAAACCTACGAGAAGTGGGAAGCCAGGCTACGTGACAAACGAGCAAAAACCATCATCGC
AGCGCGTTTGATGCGATTGGCGGAAGGTTTGCCAGGCGATATTGAATCGGTAGGCGAAGGAGTTAACGAATTGCGGATTC
ACTACGGCCCGGGCTATCGCGTCTACTTCCAGCGACGGGGAAACCTTCTGGTCGTATTGCTGTGCGGCGGAGATAAAAGC
ACTCAGGCCCGCGACATTGCCGCCGCAAAGAAACTGGCCAAAGAATGGAGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References