Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4466529..4467026 Replicon chromosome
Accession NZ_CP081762
Organism Escherichia coli strain CX21

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J6O46_RS21985 Protein ID WP_001173343.1
Coordinates 4466529..4466702 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag J6O46_RS21990 Protein ID WP_001425728.1
Coordinates 4466730..4467026 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O46_RS21975 (4463199) 4463199..4464656 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
J6O46_RS21980 (4464893) 4464893..4466410 + 1518 WP_001295074.1 lysine--tRNA ligase -
J6O46_RS21985 (4466529) 4466529..4466702 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J6O46_RS21990 (4466730) 4466730..4467026 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J6O46_RS21995 (4467253) 4467253..4467525 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J6O46_RS22000 (4467537) 4467537..4467767 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
J6O46_RS22005 (4467948) 4467948..4469579 + 1632 WP_001216486.1 sensor histidine kinase -
J6O46_RS22010 (4469576) 4469576..4470295 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4440221..4467026 26805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T213325 WP_001173343.1 NZ_CP081762:c4466702-4466529 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T213325 NZ_CP107548:232630-232905 [Pusillimonas sp. M17]
TTGCATTACGGTCTAGAAAAACGCCCCGAAGCCACGCGGCTGCATGCCGCCGCCGCCGAGTTCCTGATGCGGGTCGATAC
CCTGGCTTGGGACAATGCGGTAGCCGAAACCTACGGCCAGATGCGCAACCAACTCGAACGAAACGGGCGTGTGCTGGGCT
CGCTGGACATGTTGATCGCAGCCCATGCGCTGCACACCGGGTCGGTGCTGGTTACTAACGACCAAGCGTTTGCGGCGATA
CCGGGCCTGGGTATAGAGGATTGGACCGGGGCATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT213325 WP_001425728.1 NZ_CP081762:c4467026-4466730 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT213325 NZ_CP107548:232245-232505 [Pusillimonas sp. M17]
ATGAGCCACGTCGCTAAGCTATTCATTAACGGTCGCAGGCGAGCCGTGCGGTTGCCGGCCGCCTATCGATTTGACACCAA
AGAGGTCTTCATACGTAAAGATCCGGAAACAGGCGACGCCATTCTGTCACGCAGGCCAGAGACATGGGACAGTTTCTTTC
GCACAATAAAGGACAAGGTCGGGCAAGACAGCTTCCTGGGCAATACGGAACGCGACCAGAAGCAGGGCCACCGGGATCCA
TTCAAGGAATGGCGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References