Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4252584..4253160 Replicon chromosome
Accession NZ_CP081762
Organism Escherichia coli strain CX21

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag J6O46_RS20905 Protein ID WP_001297643.1
Coordinates 4252873..4253160 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag J6O46_RS20900 Protein ID WP_000063160.1
Coordinates 4252584..4252886 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O46_RS20885 (4249180) 4249180..4251330 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
J6O46_RS20890 (4251380) 4251380..4251583 + 204 WP_000467859.1 YbdD/YjiX family protein -
J6O46_RS20895 (4251594) 4251594..4252550 + 957 WP_001297640.1 GTPase -
J6O46_RS20900 (4252584) 4252584..4252886 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
J6O46_RS20905 (4252873) 4252873..4253160 - 288 WP_001297643.1 BrnT family toxin Toxin
J6O46_RS20910 (4253359) 4253359..4254055 - 697 Protein_4102 IS1 family transposase -
J6O46_RS20915 (4254370) 4254370..4255602 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
J6O46_RS20920 (4255643) 4255643..4256923 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4253359..4253568 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T213322 WP_001297643.1 NZ_CP081762:c4253160-4252873 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T213322 NZ_CP107532:c891940-891680 [Methylobacterium sp. FF17]
ATGAAGCTCAGTGGACGACACCGCTCGACCCTGGAGGCGATCTTCGCCGAACCGGTCCGGGCCGGCATTGCCTGGAGCGA
CATCGAGGCGATGCTGACGGCCTTCGGCGCCGAAATCTCGGAAGGGCGCGGATCGCGGGTCCGGATCGCGCTGAACGGTG
TCCGGGCGGTGTTTCACCGTCCGCATCCGCAGAAGGAGACCGACAAGGGCGCGGTGAAGTCCATGAGGCGATTTCTGACC
GAAGCCGAGGCGACGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT213322 WP_000063160.1 NZ_CP081762:c4252886-4252584 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT213322 NZ_CP107532:c891683-891354 [Methylobacterium sp. FF17]
ATGACGACCATGCATTGCAAGGGCTACGAAGCCGTCGTCGAATACGACGCGGACGCCGACCTCTTTCACGGCGAAGTCAT
CGACATGCGCGACGTCGTGACCTTTCAGGGCCGATCCGTCGATGAACTCAAGCAGGCGTTCTCCGACAGCATCGACGATT
ACCTGGCGTTCTGCGCCGAGCGCGGCGAAGAACCCGAGAAACCCTATTCGGGCCAGTTCGTCGTGCGGGTGGAGCCGGTC
CTGCACCGCGCCGTGGTCTCGGCGGCCAAGCGGGCCGGGATCAGCCTGAACAAGTGGGTCGCCCGGACGCTGGAACGGGC
GACGATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References