Detailed information of TA system TA213320

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3896680..3897359
• Replicon: chromosome
• Accession: NZ_CP081762
• Organism: Escherichia coli strain CX21

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O46_RS19120
• Protein ID: WP_000854680.1
• Coordinates: 3897018..3897359 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O46_RS19115
• Protein ID: WP_000070396.1
• Coordinates: 3896680..3896997 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O46_RS19070 (3892118)	3892118..3892939	+	822	WP_000197388.1	DUF932 domain-containing protein	-
J6O46_RS19075 (3893156)	3893156..3893857	+	702	WP_060616110.1	WYL domain-containing protein	-
J6O46_RS19080 (3893898)	3893898..3894134	+	237	WP_001144031.1	protein YpjK	-
J6O46_RS19085 (3894134)	3894134..3894577	+	444	WP_000649865.1	hypothetical protein	-
J6O46_RS19090 (3894600)	3894600..3895067	+	468	WP_001385283.1	protein YkfB	-
J6O46_RS19095 (3895144)	3895144..3895383	+	240	WP_000194654.1	DUF905 family protein	-
J6O46_RS19100 (3895481)	3895481..3895939	+	459	WP_000211838.1	antirestriction protein	-
J6O46_RS19105 (3895955)	3895955..3896431	+	477	WP_001549015.1	RadC family protein	-
J6O46_RS19110 (3896440)	3896440..3896661	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O46_RS19115 (3896680)	3896680..3896997	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O46_RS19120 (3897018)	3897018..3897359	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O46_RS19125 (3898180)	3898180..3899646	-	1467	WP_000723924.1	hypothetical protein	-
J6O46_RS19130 (3899962)	3899962..3900351	-	390	WP_223596848.1	hypothetical protein	-
J6O46_RS19135 (3900595)	3900595..3900681	-	87	Protein_3758	capsid protein	-
J6O46_RS19140 (3900886)	3900886..3901659	+	774	WP_000355484.1	hypothetical protein	-
J6O46_RS19145 (3901720)	3901720..3902274	-	555	WP_000905043.1	site-specific DNA recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3892118..3936373	44255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T213320 WP_000854680.1 NZ_CP081762:3897018-3897359 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T213320 NZ_CP107524:1472971-1473816 [Streptococcus agalactiae]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT213320 WP_000070396.1 NZ_CP081762:3896680-3896997 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT213320 NZ_CP107524:1472384-1472974 [Streptococcus agalactiae]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGCATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAACATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7