Detailed information of TA system TA213299

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 12124..12803
• Replicon: plasmid unnamed5
• Accession: NZ_CP081745
• Organism: Escherichia coli strain CX15

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O52_RS25595
• Protein ID: WP_000854680.1
• Coordinates: 12124..12465 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O52_RS25600
• Protein ID: WP_000070396.1
• Coordinates: 12486..12803 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O52_RS25570 (J6O52_25495)	7212..7766	+	555	WP_000905043.1	site-specific DNA recombinase	-
J6O52_RS25575 (J6O52_25500)	7827..8599	-	773	Protein_11	hypothetical protein	-
J6O52_RS25580	8804..8890	+	87	Protein_12	capsid protein	-
J6O52_RS25585 (J6O52_25505)	9134..9523	+	390	WP_223596848.1	hypothetical protein	-
J6O52_RS25590 (J6O52_25510)	9839..11305	+	1467	WP_000723924.1	hypothetical protein	-
J6O52_RS25595 (J6O52_25515)	12124..12465	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O52_RS25600 (J6O52_25520)	12486..12803	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O52_RS25605 (J6O52_25525)	12822..13043	-	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O52_RS25610 (J6O52_25530)	13052..13529	-	478	Protein_18	RadC family protein	-
J6O52_RS25615 (J6O52_25535)	13545..14003	-	459	WP_000211838.1	antirestriction protein	-
J6O52_RS25620 (J6O52_25540)	14101..14340	-	240	WP_000194654.1	DUF905 family protein	-
J6O52_RS25625 (J6O52_25545)	14418..14885	-	468	WP_001385283.1	protein YkfB	-
J6O52_RS25630 (J6O52_25550)	14908..15351	-	444	WP_223617416.1	hypothetical protein	-
J6O52_RS25635 (J6O52_25555)	15351..15587	-	237	WP_001144031.1	protein YpjK	-
J6O52_RS25640 (J6O52_25560)	15628..16329	-	702	Protein_24	WYL domain-containing protein	-
J6O52_RS25645 (J6O52_25565)	16548..17365	-	818	Protein_25	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18368	18368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T213299 WP_000854680.1 NZ_CP081745:c12465-12124 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T213299 NZ_CP107518:c27007-25685 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT213299 WP_000070396.1 NZ_CP081745:c12803-12486 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT213299 NZ_CP107518:c27273-27007 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7