Detailed information of TA system TA213298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 78706..79307
• Replicon: plasmid unnamed2
• Accession: NZ_CP081744
• Organism: Escherichia coli strain CX15

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: J6O52_RS25440
• Protein ID: WP_001216034.1
• Coordinates: 78706..79086 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: J6O52_RS25445
• Protein ID: WP_001190712.1
• Coordinates: 79086..79307 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O52_RS25405 (J6O52_25320)	74213..74458	-	246	Protein_94	phage antirepressor KilAC domain-containing protein	-
J6O52_RS25410 (J6O52_25325)	74494..75078	-	585	Protein_95	transposase	-
J6O52_RS25415 (J6O52_25330)	75131..75835	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O52_RS25420 (J6O52_25335)	75901..76824	-	924	Protein_97	terminase	-
J6O52_RS25425 (J6O52_25340)	76910..77362	-	453	WP_053263817.1	Late promoter-activating protein	-
J6O52_RS25430 (J6O52_25345)	77451..78494	-	1044	WP_054622888.1	DUF968 domain-containing protein	-
J6O52_RS25435 (J6O52_25350)	78522..78701	-	180	WP_001339207.1	hypothetical protein	-
J6O52_RS25440 (J6O52_25355)	78706..79086	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
J6O52_RS25445 (J6O52_25360)	79086..79307	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J6O52_RS25450 (J6O52_25365)	79474..80178	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
J6O52_RS25455 (J6O52_25370)	80234..80530	-	297	Protein_104	IS481 family transposase	-
J6O52_RS25460 (J6O52_25375)	80582..81286	-	705	WP_001572363.1	IS6-like element IS26 family transposase	-
J6O52_RS25465 (J6O52_25380)	81440..82462	+	1023	WP_001572362.1	IS110 family transposase	-
J6O52_RS25470 (J6O52_25385)	83508..83838	+	331	Protein_107	type II toxin-antitoxin system RelE/ParE family toxin	-
J6O52_RS25475 (J6O52_25390)	83819..84100	+	282	WP_000780222.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..94675	94675
	-	inside	IScluster/Tn	-	-	74494..84881	10387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T213298 WP_001216034.1 NZ_CP081744:c79086-78706 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T213298 NZ_CP107517:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT213298 WP_001190712.1 NZ_CP081744:c79307-79086 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT213298 NZ_CP107517:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6