Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 45630..46273 Replicon plasmid unnamed2
Accession NZ_CP081744
Organism Escherichia coli strain CX15

Toxin (Protein)


Gene name vagD Uniprot ID A0A6N9SI53
Locus tag J6O52_RS25230 Protein ID WP_032192044.1
Coordinates 45857..46273 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag J6O52_RS25225 Protein ID WP_001261283.1
Coordinates 45630..45860 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O52_RS25195 (J6O52_25105) 40900..41451 + 552 WP_223610888.1 hypothetical protein -
J6O52_RS25200 (J6O52_25110) 41485..42182 + 698 WP_223610889.1 IS1 family transposase -
J6O52_RS25205 (J6O52_25115) 42328..43122 - 795 WP_016240356.1 site-specific integrase -
J6O52_RS25210 (J6O52_25120) 43320..44335 - 1016 Protein_55 hypothetical protein -
J6O52_RS25215 (J6O52_25125) 44505..44810 - 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB -
J6O52_RS25220 (J6O52_25130) 44812..45030 - 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA -
J6O52_RS25225 (J6O52_25135) 45630..45860 + 231 WP_001261283.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O52_RS25230 (J6O52_25140) 45857..46273 + 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O52_RS25235 (J6O52_25145) 46309..46560 + 252 WP_105966327.1 hypothetical protein -
J6O52_RS25240 (J6O52_25150) 46641..47741 - 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
J6O52_RS25245 (J6O52_25155) 49063..50406 - 1344 WP_032192047.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..94675 94675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15113.64 Da        Isoelectric Point: 7.7809

>T213297 WP_032192044.1 NZ_CP081744:45857-46273 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLTRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATIEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T213297 NZ_CP107516:c9979-9665 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT213297 WP_001261283.1 NZ_CP081744:45630-45860 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT213297 NZ_CP107516:c10245-9967 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9SI53

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References