Detailed information of TA system TA213296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 44505..45030
• Replicon: plasmid unnamed2
• Accession: NZ_CP081744
• Organism: Escherichia coli strain CX15

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O52_RS25215
• Protein ID: WP_016240353.1
• Coordinates: 44505..44810 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A8H9ZZC6
• Locus tag: J6O52_RS25220
• Protein ID: WP_016240352.1
• Coordinates: 44812..45030 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O52_RS25195 (J6O52_25105)	40900..41451	+	552	WP_223610888.1	hypothetical protein	-
J6O52_RS25200 (J6O52_25110)	41485..42182	+	698	WP_223610889.1	IS1 family transposase	-
J6O52_RS25205 (J6O52_25115)	42328..43122	-	795	WP_016240356.1	site-specific integrase	-
J6O52_RS25210 (J6O52_25120)	43320..44335	-	1016	Protein_55	hypothetical protein	-
J6O52_RS25215 (J6O52_25125)	44505..44810	-	306	WP_016240353.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O52_RS25220 (J6O52_25130)	44812..45030	-	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O52_RS25225 (J6O52_25135)	45630..45860	+	231	WP_001261283.1	type II toxin-antitoxin system VapB family antitoxin	-
J6O52_RS25230 (J6O52_25140)	45857..46273	+	417	WP_032192044.1	type II toxin-antitoxin system VapC family toxin	-
J6O52_RS25235 (J6O52_25145)	46309..46560	+	252	WP_105966327.1	hypothetical protein	-
J6O52_RS25240 (J6O52_25150)	46641..47741	-	1101	WP_032192045.1	NADH:flavin oxidoreductase/NADH oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..94675	94675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11518.20 Da
• Isoelectric Point: 5.6752

>T213296 WP_016240353.1 NZ_CP081744:c44810-44505 [Escherichia coli]
MQFKVYAYKRESCYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213296 NZ_CP107515:c91481-91065 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8321.25 Da
• Isoelectric Point: 4.5358

>AT213296 WP_016240352.1 NZ_CP081744:c45030-44812 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Nucleotide

• Length: 219 bp

>AT213296 NZ_CP107515:c91708-91478 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA