Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54201..54844 Replicon plasmid unnamed1
Accession NZ_CP081743
Organism Escherichia coli strain CX15

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag J6O52_RS24565 Protein ID WP_001044765.1
Coordinates 54201..54617 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O52_RS24570 Protein ID WP_001261282.1
Coordinates 54614..54844 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O52_RS24535 (J6O52_24455) 49460..49945 + 486 WP_000045622.1 conjugative transfer protein MobI(A/C) -
J6O52_RS24540 (J6O52_24460) 49942..50307 + 366 WP_001515713.1 hypothetical protein -
J6O52_RS24545 (J6O52_24465) 50408..50629 - 222 Protein_58 IS5/IS1182 family transposase -
J6O52_RS24550 (J6O52_24470) 50683..51377 + 695 WP_085949432.1 IS1-like element IS1N family transposase -
J6O52_RS24555 (J6O52_24475) 51558..52580 - 1023 WP_000361404.1 helicase UvrD -
J6O52_RS24560 (J6O52_24480) 52565..54127 - 1563 WP_001128475.1 AAA family ATPase -
J6O52_RS24565 (J6O52_24485) 54201..54617 - 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O52_RS24570 (J6O52_24490) 54614..54844 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O52_RS24575 (J6O52_24495) 54801..55223 + 423 WP_162534719.1 hypothetical protein -
J6O52_RS24580 (J6O52_24500) 55407..55769 + 363 WP_000533745.1 hypothetical protein -
J6O52_RS24585 (J6O52_24505) 55787..56596 + 810 WP_000654269.1 hypothetical protein -
J6O52_RS24590 (J6O52_24510) 56900..57880 + 981 WP_016236303.1 IS5-like element ISKpn26 family transposase -
J6O52_RS24595 (J6O52_24515) 57994..58131 + 138 WP_223610884.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146653 146653
- flank IS/Tn - - 56900..57880 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T213295 WP_001044765.1 NZ_CP081743:c54617-54201 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T213295 NZ_CP107515:c78392-78243 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213295 WP_001261282.1 NZ_CP081743:c54844-54614 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213295 NZ_CP107515:78437-78496 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References