Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4114391..4114941 Replicon chromosome
Accession NZ_CP081742
Organism Escherichia coli strain CX15

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag J6O52_RS20150 Protein ID WP_001160971.1
Coordinates 4114391..4114705 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J6O52_RS20155 Protein ID WP_000125566.1
Coordinates 4114708..4114941 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O52_RS20125 (4110066) 4110066..4111352 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J6O52_RS20130 (4111352) 4111352..4112341 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J6O52_RS20135 (4112338) 4112338..4113159 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J6O52_RS20140 (4113162) 4113162..4113539 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J6O52_RS20145 (4113546) 4113546..4114394 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J6O52_RS20150 (4114391) 4114391..4114705 - 315 WP_001160971.1 CcdB family protein Toxin
J6O52_RS20155 (4114708) 4114708..4114941 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6O52_RS20160 (4115027) 4115027..4115506 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
J6O52_RS20165 (4115698) 4115698..4117560 - 1863 WP_223610817.1 glutathione-regulated potassium-efflux system protein KefC -
J6O52_RS20170 (4117553) 4117553..4118083 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J6O52_RS20175 (4118194) 4118194..4119525 - 1332 WP_001183198.1 MFS transporter -
J6O52_RS20180 (4119583) 4119583..4119870 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T213289 WP_001160971.1 NZ_CP081742:c4114705-4114391 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T213289 NZ_CP107513:3081230-3081577 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT213289 WP_000125566.1 NZ_CP081742:c4114941-4114708 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT213289 NZ_CP107513:3081577-3081876 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References