Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3667637..3668255 Replicon chromosome
Accession NZ_CP081742
Organism Escherichia coli strain CX15

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J6O52_RS17900 Protein ID WP_001291435.1
Coordinates 3668037..3668255 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J6O52_RS17895 Protein ID WP_000344800.1
Coordinates 3667637..3668011 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O52_RS17885 (3662726) 3662726..3663919 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6O52_RS17890 (3663942) 3663942..3667091 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J6O52_RS17895 (3667637) 3667637..3668011 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J6O52_RS17900 (3668037) 3668037..3668255 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J6O52_RS17905 (3668427) 3668427..3668978 + 552 WP_000102564.1 maltose O-acetyltransferase -
J6O52_RS17910 (3669094) 3669094..3669564 + 471 WP_000136192.1 YlaC family protein -
J6O52_RS17915 (3669728) 3669728..3671278 + 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J6O52_RS17920 (3671320) 3671320..3671673 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J6O52_RS17930 (3672052) 3672052..3672363 + 312 WP_000409911.1 MGMT family protein -
J6O52_RS17935 (3672394) 3672394..3672966 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213287 WP_001291435.1 NZ_CP081742:3668037-3668255 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T213287 NZ_CP107513:c2649698-2649315 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT213287 WP_000344800.1 NZ_CP081742:3667637-3668011 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT213287 NZ_CP107513:c2649940-2649698 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References