Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54267..54910 Replicon plasmid unnamed2
Accession NZ_CP081726
Organism Escherichia coli strain CX07

Toxin (Protein)


Gene name vagD Uniprot ID A0A6N9SI53
Locus tag J6O12_RS25260 Protein ID WP_032192044.1
Coordinates 54494..54910 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag J6O12_RS25255 Protein ID WP_001261283.1
Coordinates 54267..54497 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O12_RS25225 (J6O12_25130) 49537..50088 + 552 WP_223610888.1 hypothetical protein -
J6O12_RS25230 (J6O12_25135) 50122..50819 + 698 WP_223610889.1 IS1 family transposase -
J6O12_RS25235 (J6O12_25140) 50965..51759 - 795 WP_016240356.1 site-specific integrase -
J6O12_RS25240 (J6O12_25145) 51957..52972 - 1016 Protein_61 hypothetical protein -
J6O12_RS25245 (J6O12_25150) 53142..53447 - 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB -
J6O12_RS25250 (J6O12_25155) 53449..53667 - 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA -
J6O12_RS25255 (J6O12_25160) 54267..54497 + 231 WP_001261283.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O12_RS25260 (J6O12_25165) 54494..54910 + 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O12_RS25265 (J6O12_25170) 54946..55197 + 252 WP_105966327.1 hypothetical protein -
J6O12_RS25270 (J6O12_25175) 55278..56378 - 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
J6O12_RS25275 (J6O12_25180) 57700..59043 - 1344 WP_032192047.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..94676 94676


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15113.64 Da        Isoelectric Point: 7.7809

>T213266 WP_032192044.1 NZ_CP081726:54494-54910 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLTRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATIEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T213266 NZ_CP107508:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT213266 WP_001261283.1 NZ_CP081726:54267-54497 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT213266 NZ_CP107508:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9SI53

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References