Detailed information of TA system TA213265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53142..53667
• Replicon: plasmid unnamed2
• Accession: NZ_CP081726
• Organism: Escherichia coli strain CX07

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O12_RS25245
• Protein ID: WP_016240353.1
• Coordinates: 53142..53447 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A8H9ZZC6
• Locus tag: J6O12_RS25250
• Protein ID: WP_016240352.1
• Coordinates: 53449..53667 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O12_RS25225 (J6O12_25130)	49537..50088	+	552	WP_223610888.1	hypothetical protein	-
J6O12_RS25230 (J6O12_25135)	50122..50819	+	698	WP_223610889.1	IS1 family transposase	-
J6O12_RS25235 (J6O12_25140)	50965..51759	-	795	WP_016240356.1	site-specific integrase	-
J6O12_RS25240 (J6O12_25145)	51957..52972	-	1016	Protein_61	hypothetical protein	-
J6O12_RS25245 (J6O12_25150)	53142..53447	-	306	WP_016240353.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O12_RS25250 (J6O12_25155)	53449..53667	-	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O12_RS25255 (J6O12_25160)	54267..54497	+	231	WP_001261283.1	type II toxin-antitoxin system VapB family antitoxin	-
J6O12_RS25260 (J6O12_25165)	54494..54910	+	417	WP_032192044.1	type II toxin-antitoxin system VapC family toxin	-
J6O12_RS25265 (J6O12_25170)	54946..55197	+	252	WP_105966327.1	hypothetical protein	-
J6O12_RS25270 (J6O12_25175)	55278..56378	-	1101	WP_032192045.1	NADH:flavin oxidoreductase/NADH oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..94676	94676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11518.20 Da
• Isoelectric Point: 5.6752

>T213265 WP_016240353.1 NZ_CP081726:c53447-53142 [Escherichia coli]
MQFKVYAYKRESCYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213265 NZ_CP107508:328021-328389 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8321.25 Da
• Isoelectric Point: 4.5358

>AT213265 WP_016240352.1 NZ_CP081726:c53667-53449 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Nucleotide

• Length: 219 bp

>AT213265 NZ_CP107508:327803-328024 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA