Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 107168..107811 Replicon plasmid unnamed1
Accession NZ_CP081725
Organism Escherichia coli strain CX07

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag J6O12_RS24820 Protein ID WP_001044765.1
Coordinates 107168..107584 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O12_RS24825 Protein ID WP_001261282.1
Coordinates 107581..107811 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O12_RS24790 (J6O12_24705) 102427..102912 + 486 WP_000045622.1 conjugative transfer protein MobI(A/C) -
J6O12_RS24795 (J6O12_24710) 102909..103274 + 366 WP_001515713.1 hypothetical protein -
J6O12_RS24800 (J6O12_24715) 103375..103596 - 222 Protein_109 IS5/IS1182 family transposase -
J6O12_RS24805 (J6O12_24720) 103650..104344 + 695 WP_085949432.1 IS1-like element IS1N family transposase -
J6O12_RS24810 (J6O12_24725) 104525..105547 - 1023 WP_000361404.1 helicase UvrD -
J6O12_RS24815 (J6O12_24730) 105532..107094 - 1563 WP_001128475.1 AAA family ATPase -
J6O12_RS24820 (J6O12_24735) 107168..107584 - 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O12_RS24825 (J6O12_24740) 107581..107811 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O12_RS24830 (J6O12_24745) 107768..108190 + 423 WP_162534719.1 hypothetical protein -
J6O12_RS24835 (J6O12_24750) 108374..108736 + 363 WP_000533745.1 hypothetical protein -
J6O12_RS24840 (J6O12_24755) 108754..109563 + 810 WP_000654269.1 hypothetical protein -
J6O12_RS24845 (J6O12_24760) 109867..110847 + 981 WP_016236303.1 IS5-like element ISKpn26 family transposase -
J6O12_RS24850 (J6O12_24765) 110961..111098 + 138 WP_223610884.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146661 146661
- flank IS/Tn - - 109867..110847 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T213264 WP_001044765.1 NZ_CP081725:c107584-107168 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T213264 NZ_CP107507:2292-2558 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213264 WP_001261282.1 NZ_CP081725:c107811-107581 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213264 NZ_CP107507:2084-2308 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References