Detailed information of TA system TA213260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4298036..4298631
• Replicon: chromosome
• Accession: NZ_CP081724
• Organism: Escherichia coli strain CX07

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: J6O12_RS21050
• Protein ID: WP_000239581.1
• Coordinates: 4298036..4298386 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: J6O12_RS21055
• Protein ID: WP_001223213.1
• Coordinates: 4298380..4298631 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O12_RS21030 (4293485)	4293485..4294507	-	1023	WP_001548970.1	ABC transporter permease	-
J6O12_RS21035 (4294521)	4294521..4296023	-	1503	WP_001095667.1	sugar ABC transporter ATP-binding protein	-
J6O12_RS21040 (4296161)	4296161..4297117	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
J6O12_RS21045 (4297427)	4297427..4297957	+	531	WP_000055072.1	inorganic diphosphatase	-
J6O12_RS21050 (4298036)	4298036..4298386	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
J6O12_RS21055 (4298380)	4298380..4298631	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
J6O12_RS21060 (4298843)	4298843..4299184	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
J6O12_RS21065 (4299187)	4299187..4302966	-	3780	WP_001548968.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T213260 WP_000239581.1 NZ_CP081724:c4298386-4298036 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T213260 NZ_CP107504:c149697-149206 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT213260 WP_001223213.1 NZ_CP081724:c4298631-4298380 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT213260 NZ_CP107504:c149951-149685 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7