Detailed information of TA system TA213236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 41010..41535
• Replicon: plasmid unnamed2
• Accession: NZ_CP081721
• Organism: Escherichia coli strain CX05

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O38_RS25220
• Protein ID: WP_016240353.1
• Coordinates: 41230..41535 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A8H9ZZC6
• Locus tag: J6O38_RS25215
• Protein ID: WP_016240352.1
• Coordinates: 41010..41228 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O38_RS25195 (J6O38_25105)	38299..39399	+	1101	WP_032192045.1	NADH:flavin oxidoreductase/NADH oxidase	-
J6O38_RS25200 (J6O38_25110)	39480..39731	-	252	WP_105966327.1	hypothetical protein	-
J6O38_RS25205 (J6O38_25115)	39767..40183	-	417	WP_032192044.1	type II toxin-antitoxin system VapC family toxin	-
J6O38_RS25210 (J6O38_25120)	40180..40410	-	231	WP_001261283.1	type II toxin-antitoxin system VapB family antitoxin	-
J6O38_RS25215 (J6O38_25125)	41010..41228	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O38_RS25220 (J6O38_25130)	41230..41535	+	306	WP_016240353.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O38_RS25225 (J6O38_25135)	41705..42720	+	1016	Protein_56	hypothetical protein	-
J6O38_RS25230 (J6O38_25140)	42918..43712	+	795	WP_016240356.1	site-specific integrase	-
J6O38_RS25240 (J6O38_25150)	44589..45140	-	552	WP_223610888.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..94675	94675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11518.20 Da
• Isoelectric Point: 5.6752

>T213236 WP_016240353.1 NZ_CP081721:41230-41535 [Escherichia coli]
MQFKVYAYKRESCYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213236 NZ_CP107498:3029710-3030078 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8321.25 Da
• Isoelectric Point: 4.5358

>AT213236 WP_016240352.1 NZ_CP081721:41010-41228 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Nucleotide

• Length: 219 bp

>AT213236 NZ_CP107498:3029492-3029713 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA