Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 39767..40410 Replicon plasmid unnamed2
Accession NZ_CP081721
Organism Escherichia coli strain CX05

Toxin (Protein)


Gene name vagD Uniprot ID A0A6N9SI53
Locus tag J6O38_RS25205 Protein ID WP_032192044.1
Coordinates 39767..40183 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag J6O38_RS25210 Protein ID WP_001261283.1
Coordinates 40180..40410 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O38_RS25190 (J6O38_25100) 35634..36977 + 1344 WP_032192047.1 APC family permease -
J6O38_RS25195 (J6O38_25105) 38299..39399 + 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
J6O38_RS25200 (J6O38_25110) 39480..39731 - 252 WP_105966327.1 hypothetical protein -
J6O38_RS25205 (J6O38_25115) 39767..40183 - 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O38_RS25210 (J6O38_25120) 40180..40410 - 231 WP_001261283.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O38_RS25215 (J6O38_25125) 41010..41228 + 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA -
J6O38_RS25220 (J6O38_25130) 41230..41535 + 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB -
J6O38_RS25225 (J6O38_25135) 41705..42720 + 1016 Protein_56 hypothetical protein -
J6O38_RS25230 (J6O38_25140) 42918..43712 + 795 WP_016240356.1 site-specific integrase -
J6O38_RS25240 (J6O38_25150) 44589..45140 - 552 WP_223610888.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..94675 94675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15113.64 Da        Isoelectric Point: 7.7809

>T213235 WP_032192044.1 NZ_CP081721:c40183-39767 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLTRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATIEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T213235 NZ_CP107498:c2624149-2623766 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT213235 WP_001261283.1 NZ_CP081721:c40410-40180 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT213235 NZ_CP107498:c2624391-2624149 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9SI53

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References