Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 77655..78298 Replicon plasmid unnamed1
Accession NZ_CP081720
Organism Escherichia coli strain CX05

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag J6O38_RS24570 Protein ID WP_001044765.1
Coordinates 77882..78298 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O38_RS24565 Protein ID WP_001261282.1
Coordinates 77655..77885 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O38_RS24540 (J6O38_24460) 74368..74505 - 138 WP_223610884.1 hypothetical protein -
J6O38_RS24545 (J6O38_24465) 74619..75599 - 981 WP_016236303.1 IS5-like element ISKpn26 family transposase -
J6O38_RS24550 (J6O38_24470) 75903..76712 - 810 WP_000654269.1 hypothetical protein -
J6O38_RS24555 (J6O38_24475) 76730..77092 - 363 WP_000533745.1 hypothetical protein -
J6O38_RS24560 (J6O38_24480) 77276..77698 - 423 WP_162534719.1 hypothetical protein -
J6O38_RS24565 (J6O38_24485) 77655..77885 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O38_RS24570 (J6O38_24490) 77882..78298 + 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O38_RS24575 (J6O38_24495) 78372..79934 + 1563 WP_001128475.1 AAA family ATPase -
J6O38_RS24580 (J6O38_24500) 79919..80941 + 1023 WP_000361404.1 helicase UvrD -
J6O38_RS24590 (J6O38_24510) 81870..82091 + 222 Protein_67 IS5/IS1182 family transposase -
J6O38_RS24595 (J6O38_24515) 82192..82557 - 366 WP_001515713.1 hypothetical protein -
J6O38_RS24600 (J6O38_24520) 82554..83039 - 486 WP_000045622.1 conjugative transfer protein MobI(A/C) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146658 146658
- flank IS/Tn - - 74619..75599 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T213232 WP_001044765.1 NZ_CP081720:77882-78298 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T213232 NZ_CP107498:c2041449-2040916 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213232 WP_001261282.1 NZ_CP081720:77655-77885 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213232 NZ_CP107498:c2041726-2041460 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References