Detailed information of TA system TA213230

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4424112..4424905
• Replicon: chromosome
• Accession: NZ_CP081719
• Organism: Escherichia coli strain CX05

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: J6O38_RS21720
• Protein ID: WP_000854726.1
• Coordinates: 4424528..4424905 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: J6O38_RS21715
• Protein ID: WP_001548930.1
• Coordinates: 4424112..4424438 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O38_RS21670 (4419550)	4419550..4420371	+	822	WP_223610821.1	DUF932 domain-containing protein	-
J6O38_RS21675 (4420588)	4420588..4421289	+	702	WP_016239437.1	WYL domain-containing protein	-
J6O38_RS21680 (4421330)	4421330..4421566	+	237	WP_001144031.1	protein YpjK	-
J6O38_RS21685 (4421566)	4421566..4422009	+	444	WP_001547764.1	lipoprotein YafY	-
J6O38_RS21690 (4422032)	4422032..4422499	+	468	WP_001385283.1	protein YkfB	-
J6O38_RS21695 (4422576)	4422576..4422815	+	240	WP_000194654.1	DUF905 family protein	-
J6O38_RS21700 (4422913)	4422913..4423371	+	459	WP_000211838.1	antirestriction protein	-
J6O38_RS21705 (4423387)	4423387..4423863	+	477	WP_000811693.1	RadC family protein	-
J6O38_RS21710 (4423872)	4423872..4424093	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O38_RS21715 (4424112)	4424112..4424438	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O38_RS21720 (4424528)	4424528..4424905	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
J6O38_RS21725 (4424902)	4424902..4425390	+	489	WP_001547769.1	DUF5983 family protein	-
J6O38_RS21730 (4425402)	4425402..4425599	+	198	WP_000839269.1	DUF957 domain-containing protein	-
J6O38_RS21735 (4425684)	4425684..4426526	+	843	WP_001547770.1	DUF4942 domain-containing protein	-
J6O38_RS21740 (4427275)	4427275..4428813	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4409821..4436626	26805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T213230 WP_000854726.1 NZ_CP081719:4424528-4424905 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T213230 NZ_CP107497:2292-2558 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT213230 WP_001548930.1 NZ_CP081719:4424112-4424438 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT213230 NZ_CP107497:2084-2308 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7