Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4222184..4222760 Replicon chromosome
Accession NZ_CP081719
Organism Escherichia coli strain CX05

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag J6O38_RS20685 Protein ID WP_001297643.1
Coordinates 4222473..4222760 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag J6O38_RS20680 Protein ID WP_000063160.1
Coordinates 4222184..4222486 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O38_RS20665 (4218780) 4218780..4220930 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
J6O38_RS20670 (4220980) 4220980..4221183 + 204 WP_000467859.1 YbdD/YjiX family protein -
J6O38_RS20675 (4221194) 4221194..4222150 + 957 WP_001297640.1 GTPase -
J6O38_RS20680 (4222184) 4222184..4222486 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
J6O38_RS20685 (4222473) 4222473..4222760 - 288 WP_001297643.1 BrnT family toxin Toxin
J6O38_RS20690 (4222959) 4222959..4223655 - 697 Protein_4057 IS1 family transposase -
J6O38_RS20695 (4223970) 4223970..4225202 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
J6O38_RS20700 (4225243) 4225243..4226523 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4222959..4223168 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T213228 WP_001297643.1 NZ_CP081719:c4222760-4222473 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T213228 NZ_CP107495:c91487-91071 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT213228 WP_000063160.1 NZ_CP081719:c4222486-4222184 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT213228 NZ_CP107495:c91714-91484 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References