Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4114374..4114924 Replicon chromosome
Accession NZ_CP081719
Organism Escherichia coli strain CX05

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag J6O38_RS20155 Protein ID WP_001160971.1
Coordinates 4114374..4114688 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J6O38_RS20160 Protein ID WP_000125566.1
Coordinates 4114691..4114924 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O38_RS20130 (4110049) 4110049..4111335 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J6O38_RS20135 (4111335) 4111335..4112324 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J6O38_RS20140 (4112321) 4112321..4113142 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J6O38_RS20145 (4113145) 4113145..4113522 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J6O38_RS20150 (4113529) 4113529..4114377 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J6O38_RS20155 (4114374) 4114374..4114688 - 315 WP_001160971.1 CcdB family protein Toxin
J6O38_RS20160 (4114691) 4114691..4114924 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6O38_RS20165 (4115010) 4115010..4115489 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
J6O38_RS20170 (4115681) 4115681..4117543 - 1863 WP_223610817.1 glutathione-regulated potassium-efflux system protein KefC -
J6O38_RS20175 (4117536) 4117536..4118066 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J6O38_RS20180 (4118177) 4118177..4119508 - 1332 WP_001183198.1 MFS transporter -
J6O38_RS20185 (4119566) 4119566..4119853 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T213227 WP_001160971.1 NZ_CP081719:c4114688-4114374 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T213227 NZ_CP107495:c78398-78249 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT213227 WP_000125566.1 NZ_CP081719:c4114924-4114691 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT213227 NZ_CP107495:78443-78502 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References