Detailed information of TA system TA213224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2631350..2631988
• Replicon: chromosome
• Accession: NZ_CP081719
• Organism: Escherichia coli strain CX05

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J6O38_RS12860
• Protein ID: WP_000813794.1
• Coordinates: 2631812..2631988 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J6O38_RS12855
• Protein ID: WP_001270286.1
• Coordinates: 2631350..2631766 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O38_RS12835 (2626502)	2626502..2627443	-	942	WP_001251313.1	ABC transporter permease	-
J6O38_RS12840 (2627444)	2627444..2628457	-	1014	WP_000220434.1	ABC transporter ATP-binding protein	-
J6O38_RS12845 (2628475)	2628475..2629620	-	1146	WP_000047430.1	ABC transporter substrate-binding protein	-
J6O38_RS12850 (2629865)	2629865..2631271	-	1407	WP_000760630.1	PLP-dependent aminotransferase family protein	-
J6O38_RS12855 (2631350)	2631350..2631766	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J6O38_RS12860 (2631812)	2631812..2631988	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J6O38_RS12865 (2632210)	2632210..2632440	+	231	WP_000494244.1	YncJ family protein	-
J6O38_RS12870 (2632532)	2632532..2634493	-	1962	WP_001488038.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J6O38_RS12875 (2634566)	2634566..2635102	-	537	WP_000429169.1	DNA-binding transcriptional regulator SutR	-
J6O38_RS12880 (2635194)	2635194..2636369	+	1176	WP_001236305.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T213224 WP_000813794.1 NZ_CP081719:c2631988-2631812 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T213224 NZ_CP107494:c49368-48886 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT213224 WP_001270286.1 NZ_CP081719:c2631766-2631350 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT213224 NZ_CP107494:c49622-49356 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• PDB: 6HPB