Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 320305..320936 Replicon chromosome
Accession NZ_CP081719
Organism Escherichia coli strain CX05

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag J6O38_RS01485 Protein ID WP_001259385.1
Coordinates 320305..320580 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J6O38_RS01490 Protein ID WP_000593555.1
Coordinates 320577..320936 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O38_RS01470 (315309) 315309..316376 + 1068 WP_000361470.1 HlyD family secretion protein -
J6O38_RS01475 (316373) 316373..319108 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
J6O38_RS01480 (319108) 319108..320232 + 1125 WP_001488398.1 ABC transporter permease -
J6O38_RS01485 (320305) 320305..320580 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
J6O38_RS01490 (320577) 320577..320936 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J6O38_RS01495 (321056) 321056..321457 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J6O38_RS01500 (321463) 321463..322269 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J6O38_RS01505 (322266) 322266..323030 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J6O38_RS01510 (323030) 323030..323863 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J6O38_RS01515 (323860) 323860..324804 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 275033..320936 45903


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T213206 WP_001259385.1 NZ_CP081719:320305-320580 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T213206 NZ_CP107488:4755101-4755203 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT213206 WP_000593555.1 NZ_CP081719:320577-320936 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT213206 NZ_CP107488:c4755210-4755065 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References