Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 40876..41519 Replicon plasmid unnamed2
Accession NZ_CP081715
Organism Escherichia coli strain CX04

Toxin (Protein)


Gene name vagD Uniprot ID A0A6N9SI53
Locus tag J6O07_RS25195 Protein ID WP_032192044.1
Coordinates 41103..41519 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID H9AC95
Locus tag J6O07_RS25190 Protein ID WP_001261283.1
Coordinates 40876..41106 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O07_RS25160 (J6O07_25070) 36146..36697 + 552 WP_223610888.1 hypothetical protein -
J6O07_RS25165 (J6O07_25075) 36731..37428 + 698 WP_223610889.1 IS1 family transposase -
J6O07_RS25170 (J6O07_25080) 37574..38368 - 795 WP_016240356.1 site-specific integrase -
J6O07_RS25175 (J6O07_25085) 38566..39581 - 1016 Protein_48 hypothetical protein -
J6O07_RS25180 (J6O07_25090) 39751..40056 - 306 WP_016240353.1 type II toxin-antitoxin system toxin CcdB -
J6O07_RS25185 (J6O07_25095) 40058..40276 - 219 WP_016240352.1 type II toxin-antitoxin system antitoxin CcdA -
J6O07_RS25190 (J6O07_25100) 40876..41106 + 231 WP_001261283.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O07_RS25195 (J6O07_25105) 41103..41519 + 417 WP_032192044.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O07_RS25200 (J6O07_25110) 41555..41806 + 252 WP_105966327.1 hypothetical protein -
J6O07_RS25205 (J6O07_25115) 41887..42987 - 1101 WP_032192045.1 NADH:flavin oxidoreductase/NADH oxidase -
J6O07_RS25210 (J6O07_25120) 44309..45652 - 1344 WP_032192047.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..94674 94674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15113.64 Da        Isoelectric Point: 7.7809

>T213204 WP_032192044.1 NZ_CP081715:41103-41519 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLTRLEQAVLRGHRIVISAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATIEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T213204 NZ_CP107488:3085743-3086090 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT213204 WP_001261283.1 NZ_CP081715:40876-41106 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFEL

Download         Length: 231 bp

>AT213204 NZ_CP107488:3086090-3086389 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9SI53

Antitoxin

Source ID Structure
AlphaFold DB A0A6X6R7C5

References