Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54210..54853 Replicon plasmid unnamed1
Accession NZ_CP081714
Organism Escherichia coli strain CX04

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag J6O07_RS24560 Protein ID WP_001044765.1
Coordinates 54210..54626 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O07_RS24565 Protein ID WP_001261282.1
Coordinates 54623..54853 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O07_RS24530 (J6O07_24455) 49469..49954 + 486 WP_000045622.1 conjugative transfer protein MobI(A/C) -
J6O07_RS24535 (J6O07_24460) 49951..50316 + 366 WP_001515713.1 hypothetical protein -
J6O07_RS24540 (J6O07_24465) 50417..50638 - 222 Protein_58 IS5/IS1182 family transposase -
J6O07_RS24545 (J6O07_24470) 50692..51386 + 695 WP_085949432.1 IS1-like element IS1N family transposase -
J6O07_RS24550 (J6O07_24475) 51567..52589 - 1023 WP_000361404.1 helicase UvrD -
J6O07_RS24555 (J6O07_24480) 52574..54136 - 1563 WP_001128475.1 AAA family ATPase -
J6O07_RS24560 (J6O07_24485) 54210..54626 - 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O07_RS24565 (J6O07_24490) 54623..54853 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O07_RS24570 (J6O07_24495) 54810..55232 + 423 WP_162534719.1 hypothetical protein -
J6O07_RS24575 (J6O07_24500) 55416..55778 + 363 WP_000533745.1 hypothetical protein -
J6O07_RS24580 (J6O07_24505) 55796..56605 + 810 WP_000654269.1 hypothetical protein -
J6O07_RS24585 (J6O07_24510) 56909..57889 + 981 WP_016236303.1 IS5-like element ISKpn26 family transposase -
J6O07_RS24590 (J6O07_24515) 58003..58140 + 138 WP_223610884.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146662 146662
- flank IS/Tn - - 56909..57889 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T213202 WP_001044765.1 NZ_CP081714:c54626-54210 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T213202 NZ_CP107488:c2654211-2653828 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213202 WP_001261282.1 NZ_CP081714:c54853-54623 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213202 NZ_CP107488:c2654453-2654211 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References