Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4436140..4436637 Replicon chromosome
Accession NZ_CP081713
Organism Escherichia coli strain CX04

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J6O07_RS21755 Protein ID WP_001173343.1
Coordinates 4436140..4436313 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag J6O07_RS21760 Protein ID WP_001425728.1
Coordinates 4436341..4436637 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O07_RS21745 (4432810) 4432810..4434267 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
J6O07_RS21750 (4434504) 4434504..4436021 + 1518 WP_001295074.1 lysine--tRNA ligase -
J6O07_RS21755 (4436140) 4436140..4436313 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J6O07_RS21760 (4436341) 4436341..4436637 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J6O07_RS21765 (4436864) 4436864..4437136 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J6O07_RS21770 (4437148) 4437148..4437378 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
J6O07_RS21775 (4437559) 4437559..4439190 + 1632 WP_001216486.1 sensor histidine kinase -
J6O07_RS21780 (4439187) 4439187..4439906 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4409832..4436637 26805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T213200 WP_001173343.1 NZ_CP081713:c4436313-4436140 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T213200 NZ_CP107488:c2173843-2173559 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT213200 WP_001425728.1 NZ_CP081713:c4436637-4436341 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT213200 NZ_CP107488:c2174075-2173833 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References