Detailed information of TA system TA213198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4298029..4298624
• Replicon: chromosome
• Accession: NZ_CP081713
• Organism: Escherichia coli strain CX04

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: J6O07_RS21045
• Protein ID: WP_000239581.1
• Coordinates: 4298029..4298379 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: J6O07_RS21050
• Protein ID: WP_001223213.1
• Coordinates: 4298373..4298624 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O07_RS21025 (4293478)	4293478..4294500	-	1023	WP_001548970.1	ABC transporter permease	-
J6O07_RS21030 (4294514)	4294514..4296016	-	1503	WP_001095667.1	sugar ABC transporter ATP-binding protein	-
J6O07_RS21035 (4296154)	4296154..4297110	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
J6O07_RS21040 (4297420)	4297420..4297950	+	531	WP_000055072.1	inorganic diphosphatase	-
J6O07_RS21045 (4298029)	4298029..4298379	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
J6O07_RS21050 (4298373)	4298373..4298624	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
J6O07_RS21055 (4298836)	4298836..4299177	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
J6O07_RS21060 (4299180)	4299180..4302959	-	3780	WP_001548968.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T213198 WP_000239581.1 NZ_CP081713:c4298379-4298029 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T213198 NZ_CP107488:1458689-1458907 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT213198 WP_001223213.1 NZ_CP081713:c4298624-4298373 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT213198 NZ_CP107488:1458288-1458662 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7