Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4114385..4114935 Replicon chromosome
Accession NZ_CP081713
Organism Escherichia coli strain CX04

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag J6O07_RS20145 Protein ID WP_001160971.1
Coordinates 4114385..4114699 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag J6O07_RS20150 Protein ID WP_000125566.1
Coordinates 4114702..4114935 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O07_RS20120 (4110060) 4110060..4111346 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
J6O07_RS20125 (4111346) 4111346..4112335 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
J6O07_RS20130 (4112332) 4112332..4113153 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
J6O07_RS20135 (4113156) 4113156..4113533 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
J6O07_RS20140 (4113540) 4113540..4114388 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
J6O07_RS20145 (4114385) 4114385..4114699 - 315 WP_001160971.1 CcdB family protein Toxin
J6O07_RS20150 (4114702) 4114702..4114935 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J6O07_RS20155 (4115021) 4115021..4115500 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
J6O07_RS20160 (4115692) 4115692..4117554 - 1863 WP_223610817.1 glutathione-regulated potassium-efflux system protein KefC -
J6O07_RS20165 (4117547) 4117547..4118077 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
J6O07_RS20170 (4118188) 4118188..4119519 - 1332 WP_001183198.1 MFS transporter -
J6O07_RS20175 (4119577) 4119577..4119864 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T213196 WP_001160971.1 NZ_CP081713:c4114699-4114385 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T213196 NZ_CP107487:2292-2558 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT213196 WP_000125566.1 NZ_CP081713:c4114935-4114702 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT213196 NZ_CP107487:2084-2308 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References