Detailed information of TA system TA213195

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3866292..3866971
• Replicon: chromosome
• Accession: NZ_CP081713
• Organism: Escherichia coli strain CX04

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O07_RS18890
• Protein ID: WP_000854680.1
• Coordinates: 3866630..3866971 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O07_RS18885
• Protein ID: WP_000070396.1
• Coordinates: 3866292..3866609 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O07_RS18840 (3861730)	3861730..3862551	+	822	WP_000197388.1	DUF932 domain-containing protein	-
J6O07_RS18845 (3862768)	3862768..3863469	+	702	WP_060616110.1	WYL domain-containing protein	-
J6O07_RS18850 (3863510)	3863510..3863746	+	237	WP_001144031.1	protein YpjK	-
J6O07_RS18855 (3863746)	3863746..3864189	+	444	WP_000649865.1	hypothetical protein	-
J6O07_RS18860 (3864212)	3864212..3864679	+	468	WP_001385283.1	protein YkfB	-
J6O07_RS18865 (3864756)	3864756..3864995	+	240	WP_000194654.1	DUF905 family protein	-
J6O07_RS18870 (3865093)	3865093..3865551	+	459	WP_000211838.1	antirestriction protein	-
J6O07_RS18875 (3865567)	3865567..3866043	+	477	WP_001549015.1	RadC family protein	-
J6O07_RS18880 (3866052)	3866052..3866273	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O07_RS18885 (3866292)	3866292..3866609	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O07_RS18890 (3866630)	3866630..3866971	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O07_RS18895 (3867792)	3867792..3869258	-	1467	WP_000723924.1	hypothetical protein	-
J6O07_RS18900 (3869574)	3869574..3869963	-	390	WP_223596848.1	hypothetical protein	-
J6O07_RS18905 (3870207)	3870207..3870293	-	87	Protein_3711	capsid protein	-
J6O07_RS18910 (3870498)	3870498..3871271	+	774	WP_000355484.1	hypothetical protein	-
J6O07_RS18915 (3871332)	3871332..3871886	-	555	WP_000905043.1	site-specific DNA recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3843354..3879709	36355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T213195 WP_000854680.1 NZ_CP081713:3866630-3866971 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T213195 NZ_CP107486:c6004-5690 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT213195 WP_000070396.1 NZ_CP081713:3866292-3866609 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT213195 NZ_CP107486:c6270-5992 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7