Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 320304..320935 Replicon chromosome
Accession NZ_CP081713
Organism Escherichia coli strain CX04

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag J6O07_RS01485 Protein ID WP_001259385.1
Coordinates 320304..320579 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J6O07_RS01490 Protein ID WP_000593555.1
Coordinates 320576..320935 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O07_RS01470 (315308) 315308..316375 + 1068 WP_000361470.1 HlyD family secretion protein -
J6O07_RS01475 (316372) 316372..319107 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
J6O07_RS01480 (319107) 319107..320231 + 1125 WP_001488398.1 ABC transporter permease -
J6O07_RS01485 (320304) 320304..320579 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
J6O07_RS01490 (320576) 320576..320935 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J6O07_RS01495 (321055) 321055..321456 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J6O07_RS01500 (321462) 321462..322268 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J6O07_RS01505 (322265) 322265..323029 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J6O07_RS01510 (323029) 323029..323862 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J6O07_RS01515 (323859) 323859..324803 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 297082..320935 23853


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T213175 WP_001259385.1 NZ_CP081713:320304-320579 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T213175 NZ_CP107479:c149601-149110 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT213175 WP_000593555.1 NZ_CP081713:320576-320935 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT213175 NZ_CP107479:c149855-149589 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References