Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 66179..66822 Replicon plasmid unnamed1
Accession NZ_CP081712
Organism Escherichia coli strain AH21

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag J6O42_RS23340 Protein ID WP_000754567.1
Coordinates 66406..66822 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag J6O42_RS23335 Protein ID WP_001261275.1
Coordinates 66179..66409 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O42_RS23310 (J6O42_23215) 62235..62932 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
J6O42_RS23315 (J6O42_23220) 63033..63314 - 282 WP_000639435.1 hypothetical protein -
J6O42_RS23320 (J6O42_23225) 63463..63708 + 246 WP_163396968.1 hypothetical protein -
J6O42_RS23325 (J6O42_23230) 63766..64554 - 789 WP_001063461.1 site-specific integrase -
J6O42_RS23330 (J6O42_23235) 64574..65245 - 672 WP_000796235.1 hypothetical protein -
J6O42_RS23335 (J6O42_23240) 66179..66409 + 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O42_RS23340 (J6O42_23245) 66406..66822 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O42_RS23345 (J6O42_23250) 67073..68119 + 1047 WP_032182506.1 IS481-like element ISKpn28 family transposase -
J6O42_RS23350 (J6O42_23255) 68111..68680 - 570 Protein_85 IS5 family transposase -
J6O42_RS23355 (J6O42_23260) 68992..69426 + 435 WP_000103648.1 RidA family protein -
J6O42_RS23360 (J6O42_23265) 69431..70105 + 675 WP_000175734.1 DUF1028 domain-containing protein -
J6O42_RS23365 (J6O42_23270) 70121..71278 + 1158 WP_000633254.1 acetylornithine deacetylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..74799 74799
- inside IScluster/Tn - - 48610..68635 20025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T213174 WP_000754567.1 NZ_CP081712:66406-66822 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T213174 NZ_CP107479:c125732-125316 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT213174 WP_001261275.1 NZ_CP081712:66179-66409 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT213174 NZ_CP107479:c125959-125729 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References