Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 35492..36093 Replicon plasmid unnamed1
Accession NZ_CP081712
Organism Escherichia coli strain AH21

Toxin (Protein)


Gene name doc Uniprot ID Q47172
Locus tag J6O42_RS23175 Protein ID WP_001694510.1
Coordinates 35713..36093 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag J6O42_RS23170 Protein ID WP_001190712.1
Coordinates 35492..35713 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O42_RS23125 (J6O42_23025) 30618..31610 + 993 WP_001145103.1 hypothetical protein -
J6O42_RS23130 (J6O42_23030) 31667..31798 + 132 Protein_40 ISNCY family transposase -
J6O42_RS23135 (J6O42_23035) 31795..32406 - 612 WP_000176305.1 DUF2913 family protein -
J6O42_RS23140 (J6O42_23040) 32460..32741 - 282 WP_001261567.1 hypothetical protein -
J6O42_RS23145 (J6O42_23045) 32923..33198 + 276 WP_223597216.1 C2H2-type zinc finger protein -
J6O42_RS23150 (J6O42_23050) 33195..33428 - 234 Protein_44 IS3 family transposase -
J6O42_RS23160 (J6O42_23060) 34779..34922 - 144 Protein_46 transposase -
J6O42_RS23165 (J6O42_23065) 34986..35309 - 324 Protein_47 type I restriction-modification system subunit M N-terminal domain-containing protein -
J6O42_RS23170 (J6O42_23070) 35492..35713 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J6O42_RS23175 (J6O42_23075) 35713..36093 + 381 WP_001694510.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J6O42_RS23180 (J6O42_23080) 36098..36277 + 180 WP_000113018.1 hypothetical protein -
J6O42_RS23185 (J6O42_23085) 36305..37348 + 1044 WP_000648832.1 DUF968 domain-containing protein -
J6O42_RS23190 (J6O42_23090) 37437..37889 + 453 WP_001326849.1 late promoter-activating protein -
J6O42_RS23195 (J6O42_23095) 37975..39168 + 1194 WP_000219610.1 hypothetical protein -
J6O42_RS23200 (J6O42_23100) 39168..40652 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..74799 74799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13616.35 Da        Isoelectric Point: 5.1514

>T213173 WP_001694510.1 NZ_CP081712:35713-36093 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T213173 NZ_CP107479:c49368-48886 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT213173 WP_001190712.1 NZ_CP081712:35492-35713 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT213173 NZ_CP107479:c49622-49356 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A737M8C8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References