Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2453515..2454153 Replicon chromosome
Accession NZ_CP081711
Organism Escherichia coli strain AH21

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J6O42_RS11815 Protein ID WP_000813794.1
Coordinates 2453977..2454153 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J6O42_RS11810 Protein ID WP_001270286.1
Coordinates 2453515..2453931 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O42_RS11790 (2448667) 2448667..2449608 - 942 WP_001251304.1 ABC transporter permease -
J6O42_RS11795 (2449609) 2449609..2450622 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
J6O42_RS11800 (2450640) 2450640..2451785 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
J6O42_RS11805 (2452030) 2452030..2453436 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J6O42_RS11810 (2453515) 2453515..2453931 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J6O42_RS11815 (2453977) 2453977..2454153 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J6O42_RS11820 (2454375) 2454375..2454605 + 231 WP_000494244.1 YncJ family protein -
J6O42_RS11825 (2454697) 2454697..2456658 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J6O42_RS11830 (2456731) 2456731..2457267 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
J6O42_RS11835 (2457359) 2457359..2458534 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T213168 WP_000813794.1 NZ_CP081711:c2454153-2453977 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T213168 NZ_CP107478:4189006-4189224 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT213168 WP_001270286.1 NZ_CP081711:c2453931-2453515 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT213168 NZ_CP107478:4188605-4188979 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References