Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2291650..2292176 Replicon chromosome
Accession NZ_CP081711
Organism Escherichia coli strain AH21

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag J6O42_RS11000 Protein ID WP_000323025.1
Coordinates 2291889..2292176 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag J6O42_RS10995 Protein ID WP_000534858.1
Coordinates 2291650..2291889 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O42_RS10965 (2286779) 2286779..2287200 + 422 Protein_2139 hypothetical protein -
J6O42_RS10970 (2287528) 2287528..2288508 + 981 Protein_2140 IS5-like element ISKpn26 family transposase -
J6O42_RS10975 (2289056) 2289056..2290078 + 1023 Protein_2141 ISNCY family transposase -
J6O42_RS10980 (2290199) 2290199..2290348 + 150 WP_011443592.1 protein YdfW -
J6O42_RS10985 (2290785) 2290785..2291117 - 333 WP_001301033.1 FlxA-like family protein -
J6O42_RS10990 (2291320) 2291320..2291625 - 306 WP_001326990.1 protein YdfV -
J6O42_RS10995 (2291650) 2291650..2291889 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
J6O42_RS11000 (2291889) 2291889..2292176 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
J6O42_RS11005 (2292248) 2292248..2292403 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
J6O42_RS11010 (2292517) 2292517..2294052 - 1536 WP_001000406.1 IS66 family transposase -
J6O42_RS11015 (2294102) 2294102..2294449 - 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -
J6O42_RS11025 (2295065) 2295065..2295316 + 252 WP_000980994.1 protein Rem -
J6O42_RS11030 (2295383) 2295383..2295661 + 279 WP_012304870.1 hypothetical protein -
J6O42_RS11035 (2295663) 2295663..2296712 + 1050 WP_001265198.1 DUF968 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2268108..2309273 41165
- inside IScluster/Tn - - 2282970..2294449 11479


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T213166 WP_000323025.1 NZ_CP081711:2291889-2292176 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T213166 NZ_CP107478:2023187-2023289 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT213166 WP_000534858.1 NZ_CP081711:2291650-2291889 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT213166 NZ_CP107478:c2023296-2023151 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References