Detailed information of TA system TA213150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 46068..46866
• Replicon: plasmid unnamed1
• Accession: NZ_CP081707
• Organism: Escherichia coli strain AH04

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: J6O21_RS24585
• Protein ID: WP_223571418.1
• Coordinates: 46068..46589 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: J6O21_RS24590
• Protein ID: WP_001351987.1
• Coordinates: 46597..46866 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O21_RS24555 (J6O21_24405)	42741..43268	+	528	WP_223571416.1	tail fiber assembly protein	-
J6O21_RS24560 (J6O21_24410)	43431..43685	+	255	WP_000120169.1	hypothetical protein	-
J6O21_RS24565	43727..44098	+	372	WP_223571417.1	hypothetical protein	-
J6O21_RS24570 (J6O21_24420)	44617..44940	+	324	WP_000064175.1	hypothetical protein	-
J6O21_RS24575 (J6O21_24425)	44954..45646	+	693	WP_096179506.1	hypothetical protein	-
J6O21_RS24580 (J6O21_24430)	45648..45899	+	252	WP_000901559.1	hypothetical protein	-
J6O21_RS24585 (J6O21_24435)	46068..46589	-	522	WP_223571418.1	GNAT family N-acetyltransferase	Toxin
J6O21_RS24590 (J6O21_24440)	46597..46866	-	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
J6O21_RS24595 (J6O21_24445)	47185..47853	+	669	WP_000161228.1	division plane positioning ATPase MipZ	-
J6O21_RS24600 (J6O21_24450)	47859..48224	+	366	WP_223571420.1	hypothetical protein	-
J6O21_RS24605 (J6O21_24455)	48271..49433	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
J6O21_RS24610 (J6O21_24460)	49512..50189	-	678	WP_223571401.1	hypothetical protein	-
J6O21_RS24615 (J6O21_24465)	50562..51302	+	741	WP_223571402.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94797	94797

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (56-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19350.07 Da
• Isoelectric Point: 8.6369

>T213150 WP_223571418.1 NZ_CP081707:c46589-46068 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERSKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSVLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T213150 NZ_CP107473:c2330660-2329743 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT213150 WP_001351987.1 NZ_CP081707:c46866-46597 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT213150 NZ_CP107473:c2331424-2330666 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7