Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 68949..69499 Replicon chromosome
Accession NC_007606
Organism Shigella dysenteriae Sd197

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag SDY_RS00345 Protein ID WP_001160974.1
Coordinates 69185..69499 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D3H431
Locus tag SDY_RS00340 Protein ID WP_000125565.1
Coordinates 68949..69182 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SDY_RS00315 64215..64502 + 288 WP_000203741.1 ferredoxin-like protein FixX -
SDY_RS00320 64560..65891 + 1332 Protein_62 MFS transporter -
SDY_RS00325 66002..66532 + 531 WP_000600719.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
SDY_RS00330 66525..68192 + 1668 WP_000377138.1 glutathione-regulated potassium-efflux system protein -
SDY_RS00335 68384..68863 + 480 WP_005020907.1 type 3 dihydrofolate reductase -
SDY_RS00340 68949..69182 + 234 WP_000125565.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
SDY_RS00345 69185..69499 + 315 WP_001160974.1 CcdB family protein Toxin
SDY_RS00350 69496..70344 - 849 WP_000257202.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
SDY_RS00355 70351..70728 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
SDY_RS00360 70731..71552 - 822 WP_001065369.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
SDY_RS00365 71549..72538 - 990 WP_000241239.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
SDY_RS00370 72538..73824 - 1287 WP_000800471.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T21315 WP_001160974.1 NC_007606:69185-69499 [Shigella dysenteriae Sd197]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T21315 NC_007606:69185-69499 [Shigella dysenteriae Sd197]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTCCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8599.64 Da        Isoelectric Point: 6.9791

>AT21315 WP_000125565.1 NC_007606:68949-69182 [Shigella dysenteriae Sd197]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Download         Length: 234 bp

>AT21315 NC_007606:68949-69182 [Shigella dysenteriae Sd197]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGCGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A090NGC7

References