Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3583545..3584163 Replicon chromosome
Accession NZ_CP081706
Organism Escherichia coli strain AH04

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J6O21_RS17810 Protein ID WP_001291435.1
Coordinates 3583945..3584163 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J6O21_RS17805 Protein ID WP_000344800.1
Coordinates 3583545..3583919 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O21_RS17795 (3578634) 3578634..3579827 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J6O21_RS17800 (3579850) 3579850..3582999 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J6O21_RS17805 (3583545) 3583545..3583919 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J6O21_RS17810 (3583945) 3583945..3584163 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J6O21_RS17815 (3584335) 3584335..3584886 + 552 WP_000102564.1 maltose O-acetyltransferase -
J6O21_RS17820 (3585002) 3585002..3585472 + 471 WP_000136192.1 YlaC family protein -
J6O21_RS17825 (3585636) 3585636..3587186 + 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J6O21_RS17830 (3587228) 3587228..3587581 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J6O21_RS17840 (3587960) 3587960..3588271 + 312 WP_000409911.1 MGMT family protein -
J6O21_RS17845 (3588302) 3588302..3588874 - 573 WP_001548252.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213145 WP_001291435.1 NZ_CP081706:3583945-3584163 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T213145 NZ_CP107472:2292-2558 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT213145 WP_000344800.1 NZ_CP081706:3583545-3583919 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT213145 NZ_CP107472:2084-2308 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References