Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2600088..2600726 Replicon chromosome
Accession NZ_CP081706
Organism Escherichia coli strain AH04

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J6O21_RS12945 Protein ID WP_000813794.1
Coordinates 2600550..2600726 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J6O21_RS12940 Protein ID WP_001270286.1
Coordinates 2600088..2600504 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O21_RS12920 (2595240) 2595240..2596181 - 942 WP_001251313.1 ABC transporter permease -
J6O21_RS12925 (2596182) 2596182..2597195 - 1014 WP_057108537.1 ABC transporter ATP-binding protein -
J6O21_RS12930 (2597213) 2597213..2598358 - 1146 Protein_2534 ABC transporter substrate-binding protein -
J6O21_RS12935 (2598603) 2598603..2600009 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J6O21_RS12940 (2600088) 2600088..2600504 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J6O21_RS12945 (2600550) 2600550..2600726 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J6O21_RS12950 (2600948) 2600948..2601178 + 231 WP_000494244.1 YncJ family protein -
J6O21_RS12955 (2601270) 2601270..2603231 - 1962 WP_001488038.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J6O21_RS12960 (2603304) 2603304..2603840 - 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
J6O21_RS12965 (2603932) 2603932..2605107 + 1176 WP_057108539.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T213144 WP_000813794.1 NZ_CP081706:c2600726-2600550 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T213144 NZ_CP107471:c4371-4057 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT213144 WP_001270286.1 NZ_CP081706:c2600504-2600088 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT213144 NZ_CP107471:c4637-4359 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References