Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2451182..2451918 Replicon chromosome
Accession NZ_CP081706
Organism Escherichia coli strain AH04

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J6O21_RS12185 Protein ID WP_003026803.1
Coordinates 2451182..2451664 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J6O21_RS12190 Protein ID WP_003026799.1
Coordinates 2451652..2451918 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O21_RS12175 (2446807) 2446807..2447268 - 462 WP_000593665.1 response regulator -
J6O21_RS12180 (2447261) 2447261..2450623 - 3363 WP_029404079.1 ATP-binding protein -
J6O21_RS12185 (2451182) 2451182..2451664 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J6O21_RS12190 (2451652) 2451652..2451918 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J6O21_RS12195 (2452363) 2452363..2452593 - 231 WP_004206660.1 hypothetical protein -
J6O21_RS12200 (2452607) 2452607..2452810 - 204 WP_004213596.1 HHA domain-containing protein -
J6O21_RS12205 (2452871) 2452871..2453362 - 492 WP_015065591.1 hypothetical protein -
J6O21_RS12210 (2453496) 2453496..2454530 - 1035 WP_015065590.1 hypothetical protein -
J6O21_RS12215 (2454832) 2454832..2455836 - 1005 WP_022542389.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T213143 WP_003026803.1 NZ_CP081706:c2451664-2451182 [Escherichia coli]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T213143 NZ_CP107470:c91481-91065 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT213143 WP_003026799.1 NZ_CP081706:c2451918-2451652 [Escherichia coli]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT213143 NZ_CP107470:c91708-91478 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References