Detailed information of TA system TA213142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2426759..2428347
• Replicon: chromosome
• Accession: NZ_CP081706
• Organism: Escherichia coli strain AH04

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A080IXU4
• Locus tag: J6O21_RS12075
• Protein ID: WP_021574605.1
• Coordinates: 2427025..2428347 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J6O21_RS12070
• Protein ID: WP_001613070.1
• Coordinates: 2426759..2427025 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O21_RS12065 (2423074)	2423074..2426601	+	3528	Protein_2360	ECSE_1600 family autotransporter	-
J6O21_RS12070 (2426759)	2426759..2427025	+	267	WP_001613070.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J6O21_RS12075 (2427025)	2427025..2428347	+	1323	WP_021574605.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J6O21_RS12080 (2428644)	2428644..2429345	-	702	WP_225247894.1	SecC motif-containing protein	-
J6O21_RS12085 (2429467)	2429467..2429907	-	441	WP_016239970.1	hypothetical protein	-
J6O21_RS12090 (2430060)	2430060..2431421	-	1362	WP_016239969.1	FRG domain-containing protein	-
J6O21_RS12095 (2431435)	2431435..2432238	-	804	WP_000589340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2432297..2433655	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49304.94 Da
• Isoelectric Point: 8.5823

>T213142 WP_021574605.1 NZ_CP081706:2427025-2428347 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T213142 NZ_CP107470:c78392-78243 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.36 Da
• Isoelectric Point: 4.9632

>AT213142 WP_001613070.1 NZ_CP081706:2426759-2427025 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT213142 NZ_CP107470:78437-78496 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A080IXU4