Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1086870..1087453 Replicon chromosome
Accession NZ_CP081706
Organism Escherichia coli strain AH04

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag J6O21_RS05430 Protein ID WP_000254739.1
Coordinates 1087118..1087453 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag J6O21_RS05425 Protein ID WP_000581937.1
Coordinates 1086870..1087118 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O21_RS05415 (1083209) 1083209..1084510 + 1302 WP_000046826.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
J6O21_RS05420 (1084558) 1084558..1086792 + 2235 WP_000226803.1 GTP diphosphokinase -
J6O21_RS05425 (1086870) 1086870..1087118 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
J6O21_RS05430 (1087118) 1087118..1087453 + 336 WP_000254739.1 endoribonuclease MazF Toxin
J6O21_RS05435 (1087524) 1087524..1088315 + 792 WP_057107940.1 nucleoside triphosphate pyrophosphohydrolase -
J6O21_RS05440 (1088543) 1088543..1090180 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
J6O21_RS05445 (1090268) 1090268..1091566 + 1299 WP_000036723.1 phosphopyruvate hydratase -
J6O21_RS05450 (1091710) 1091710..1091903 + 194 Protein_1068 transposase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12126.09 Da        Isoelectric Point: 8.2618

>T213132 WP_000254739.1 NZ_CP081706:1087118-1087453 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T213132 NZ_CP107468:2022284-2022386 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT213132 WP_000581937.1 NZ_CP081706:1086870-1087118 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT213132 NZ_CP107468:c2022393-2022248 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References