Detailed information of TA system TA213130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 870093..871661
• Replicon: chromosome
• Accession: NZ_CP081706
• Organism: Escherichia coli strain AH04

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J6O21_RS04425
• Protein ID: WP_000783857.1
• Coordinates: 870348..871661 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A859R2S1
• Locus tag: J6O21_RS04420
• Protein ID: WP_039272366.1
• Coordinates: 870093..870344 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O21_RS04385 (865319)	865319..865435	+	117	WP_000991086.1	hypothetical protein	-
J6O21_RS04390 (865748)	865748..866473	+	726	WP_001553093.1	hypothetical protein	-
J6O21_RS04395 (866529)	866529..866981	+	453	WP_079778891.1	hypothetical protein	-
J6O21_RS04400 (866997)	866997..867599	-	603	WP_039272372.1	hypothetical protein	-
J6O21_RS04405 (867860)	867860..868141	+	282	WP_000575656.1	hypothetical protein	-
J6O21_RS04410 (868160)	868160..868723	+	564	WP_223571365.1	hypothetical protein	-
J6O21_RS04415 (868726)	868726..869730	+	1005	WP_001553091.1	tyrosine-type recombinase/integrase	-
J6O21_RS04420 (870093)	870093..870344	+	252	WP_039272366.1	helix-turn-helix domain-containing protein	Antitoxin
J6O21_RS04425 (870348)	870348..871661	+	1314	WP_000783857.1	type II toxin-antitoxin system HipA family toxin	Toxin
J6O21_RS04430 (871658)	871658..872152	+	495	WP_039272364.1	hypothetical protein	-
J6O21_RS04435 (872328)	872328..873266	-	939	WP_000395773.1	hypothetical protein	-
J6O21_RS04440 (873510)	873510..875105	+	1596	WP_239605188.1	PAAR domain-containing protein	-
J6O21_RS04445 (875303)	875303..875860	-	558	WP_003100847.1	recombinase family protein	-
J6O21_RS04450 (875854)	875854..876225	-	372	WP_003100853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	849216..880941	31725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-64)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 48209.48 Da
• Isoelectric Point: 6.4189

>T213130 WP_000783857.1 NZ_CP081706:870348-871661 [Escherichia coli]
MKMQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQAR
FRVETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTA
LLWYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWS
KSGEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEP
ASSFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEV
VATVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Nucleotide

• Length: 1314 bp

>T213130 NZ_CP107468:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9369.79 Da
• Isoelectric Point: 9.8930

>AT213130 WP_039272366.1 NZ_CP081706:870093-870344 [Escherichia coli]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Nucleotide

• Length: 252 bp

>AT213130 NZ_CP107468:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A859R2S1