Detailed information of TA system TA213128

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 715797..716584
• Replicon: chromosome
• Accession: NZ_CP081706
• Organism: Escherichia coli strain AH04

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: J6O21_RS03545
• Protein ID: WP_032669201.1
• Coordinates: 716207..716584 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A6L5EE19
• Locus tag: J6O21_RS03540
• Protein ID: WP_023567998.1
• Coordinates: 715797..716156 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O21_RS03495 (711057)	711057..711770	+	714	WP_032669194.1	WYL domain-containing protein	-
J6O21_RS03500 (711767)	711767..712402	+	636	WP_020837117.1	hypothetical protein	-
J6O21_RS03505 (712441)	712441..712896	+	456	WP_000754598.1	hypothetical protein	-
J6O21_RS03510 (712893)	712893..713342	+	450	WP_032669197.1	IrmA family protein	-
J6O21_RS03515 (713414)	713414..713645	+	232	Protein_689	DUF905 domain-containing protein	-
J6O21_RS03520 (713744)	713744..714566	+	823	Protein_690	DUF945 domain-containing protein	-
J6O21_RS03525 (714597)	714597..715040	+	444	WP_032669199.1	antirestriction protein	-
J6O21_RS03530 (715052)	715052..715531	+	480	WP_032669200.1	DNA repair protein RadC	-
J6O21_RS03535 (715552)	715552..715773	+	222	WP_023567997.1	DUF987 domain-containing protein	-
J6O21_RS03540 (715797)	715797..716156	+	360	WP_023567998.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J6O21_RS03545 (716207)	716207..716584	+	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
J6O21_RS03550 (716581)	716581..717072	+	492	WP_032672023.1	DUF5983 family protein	-
J6O21_RS03555 (717102)	717102..717305	+	204	WP_032669203.1	DUF957 domain-containing protein	-
J6O21_RS03560 (717369)	717369..718217	+	849	WP_032669204.1	DUF4942 domain-containing protein	-
J6O21_RS03570 (718520)	718520..719026	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
J6O21_RS03575 (719105)	719105..720946	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T213128 WP_032669201.1 NZ_CP081706:716207-716584 [Escherichia coli]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T213128 NZ_CP107467:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13192.04 Da
• Isoelectric Point: 6.3122

>AT213128 WP_023567998.1 NZ_CP081706:715797-716156 [Escherichia coli]
MSNEIPTVNHDITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT213128 NZ_CP107467:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6

Antitoxin

• AlphaFold DB: A0A6L5EE19