Detailed information of TA system TA213118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 69046..69844
• Replicon: plasmid unnamed1
• Accession: NZ_CP081702
• Organism: Escherichia coli strain AH02

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: J6O02_RS24695
• Protein ID: WP_223571418.1
• Coordinates: 69046..69567 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: J6O02_RS24700
• Protein ID: WP_001351987.1
• Coordinates: 69575..69844 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O02_RS24665 (J6O02_24530)	65719..66246	+	528	WP_223571416.1	tail fiber assembly protein	-
J6O02_RS24670 (J6O02_24535)	66409..66663	+	255	WP_000120169.1	hypothetical protein	-
J6O02_RS24675	66705..67076	+	372	WP_223571417.1	hypothetical protein	-
J6O02_RS24680 (J6O02_24545)	67595..67918	+	324	WP_000064175.1	hypothetical protein	-
J6O02_RS24685 (J6O02_24550)	67932..68624	+	693	WP_096179506.1	hypothetical protein	-
J6O02_RS24690 (J6O02_24555)	68626..68877	+	252	WP_000901559.1	hypothetical protein	-
J6O02_RS24695 (J6O02_24560)	69046..69567	-	522	WP_223571418.1	GNAT family N-acetyltransferase	Toxin
J6O02_RS24700 (J6O02_24565)	69575..69844	-	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
J6O02_RS24705 (J6O02_24570)	70163..70831	+	669	WP_000161228.1	division plane positioning ATPase MipZ	-
J6O02_RS24710 (J6O02_24575)	70837..71202	+	366	WP_223571420.1	hypothetical protein	-
J6O02_RS24715 (J6O02_24580)	71249..72411	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
J6O02_RS24720 (J6O02_24585)	72490..73167	-	678	WP_223571401.1	hypothetical protein	-
J6O02_RS24725 (J6O02_24590)	73540..74280	+	741	WP_223571402.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94799	94799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (56-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19350.07 Da
• Isoelectric Point: 8.6369

>T213118 WP_223571418.1 NZ_CP081702:c69567-69046 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERSKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSVLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T213118 NZ_CP107463:2075152-2075254 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT213118 WP_001351987.1 NZ_CP081702:c69844-69575 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT213118 NZ_CP107463:c2075261-2075116 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7